Stains

Question 1

Wright stain

Answer 1

Contains methylene blue, stains acidic stuff blue (DNA and RNA)
Contains eosin, stains basic stuff orange (hemoglobin, eosinophilic granules)

Question 2

Prussian blue

Answer 2

Visualize iron granules

Question 3

New methylene blue

Answer 3

Precipitates RNA in reticulocytes

Question 4

Neutral red/brilliant cresyl green

Answer 4

Stain Heinz bodies

Question 5

Myeloperoxidase (MPO)

Answer 5

Stains granulocytic cells and some monocytes

Lymph’s are negative

Question 6

Sudan black B

Answer 6

Stains phospholipids and lipoproteins
Granulocytes and auer rods = positive
Lymph’s = negative

Question 7

Specific esterase

Answer 7

Granulocytes = positive 
Monocytes = negative

Question 8

Non-specific esterase

Answer 8

Monocytes = positive 
Granulocytes = negative

Question 9

Periodic acid-Schiff (PAS)

Answer 9

Stains glycogen
Immature lymphoid, malignant erythroblasts, megakaryocytic cells = positive
Myeloblasts, normal erythrocytes = negative

Question 10

Leukocyte alkaline phosphatase (LAP)

Answer 10

Grade 100 neutrophils 0-4+, add cells in each grade, multiply by grade and add up
Normal = 13-130
Decreased = CML, PNH
Normal = CML (in remission/infection), Hodgkin lymphoma (in remission), secondary polycythemia
Increased = neutrophilic leukemoid rxn, polycythemia Vera, CML (in blast crisis), late trimester pregnancy