Stains

Question 1

What antibody stain would you use for a dendritic cell?

Answer 1

CD11c+, MHC II+

Question 2

What antibody stain would you use for a T cell?

Answer 2

CD4+, CD3+

Question 3

What are some benefits of using Orcein-Giemsa stain (6)?

Answer 3

1. Stains elastic tissue black/brown 2. Stains mast cells metachromatically 3. Differentiates hemosiderin and melanin 4. Differentiates smooth muscle from collagen 5. Stains fungal elements 6. Emphasizes distinction between orthokeratotic and parakeratotic hyperkeratosis

Question 4

What is the ideal pH for Michel’s fixative?

Answer 4

7.0-7.2

Question 5

Name 2 special stains for fungi

Answer 5

PAS - periodic acid schiff

GMS - Gomori’s methanamine silver

Question 6

Name 2 stains for fats/lipids

Answer 6

Oil red O

Scarlet Red

Sudan BLack

Question 7

Name the stain for Calcium. What color?

Answer 7

Von Kossa

Stains calcium black

Question 8

Name the stain for melanin

Answer 8

Fontana Masson

Question 9

Name the stain for Amyloid

Answer 9

Alkaline Congo red

Question 10

Name the stain for collagen

Answer 10

Masson’s trichome

Stains mature collagen BLUE

Question 11

Name the tissue stain for Gram bacteria

Answer 11

Brown-Brenn

Question 12

Name 2 mast cell stains

Answer 12

Toluidine blue

Acid Orcein-Giemsa

Question 13

Name the stain for this organism

Answer 13

Mayer’s mucicarmine for cryptococcus