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TOB > Stains > Flashcards

Stains Flashcards

1
Q

What stain is used to detect mycobacterium and chylamydia?

A

Acid fast stain

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2
Q

What property does haemotoxylin have?

A

It is slightly basic
Called acidophilic
Therefore it can bind to acidic parts eg nucleic acids

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3
Q

What property does eosin have?

A

It is acidic
Called basophilic
Therefore binds to basic parts eg proteins etc

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4
Q

What is Periodic Acid Shiff used for?

What colour will they stain?

A

Staining carbohydrates and glycoproteins

They will stain pink/magenta

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5
Q

Describe the process of Gram Staining

A
  1. Add crystal violet solution (which is positively charged)
  2. Add iodine
  3. Acetone/methanol is added to wash the slide
  4. Add pink/red safranin dye
  5. Gram positive will appear purple
    Gram negative will appear pink
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TOB (10 decks)

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