Stains Flashcards
Giemsa
Chlamydia, Borrelia, Ricketsiae, Trypanosomes, Plasmodium
“Certain Bugs Really Try my Patience”
Chlamydia
Giemsa
Borrelia
Giemsa
Ricketssiae
Giemsa
Trypanosomes
Giemsa
Plasmodium
Giemsa
PAS (periodic acid-schiff)
Stains glycogen, mucoplysaccharides; used to diagnose Whipple disease (Tropheryma whipplei)
PASs the sugar
Ziehl-Neelson (carbol fuschin)
Acid-fast organisms (Nocardia, Mycobacterium), protozoa (Cryptosporidium oocysts)
Alternative is auramine-rhodamine stain for screening (inexpensive, more sensitive but less specific)
India ink
Cryptococcus neoformans (mucicarmine can also be used to stain thick polysaccharide capsule red)
Silver stain
Fungi (Pneumocystis), Legionella, Helicobacter pylori
Whipple disease
PAS
Nocardia
Ziehl-Neelson (carbol fuchsin)
Mycobacterium
Ziehl-Neelson (carbol fuchsin)
Cryptosporidium oocysts
Ziehl-Neelson (carbol fuchsin)
Cryptococcus neoformans
India ink