Culture Requirements Flashcards
H. influenzae
Chocolate agar with factors V (NAD+) and X (hematin)
N. gonorrhoeae, N. meningitidis
Thayer-Martin (or VPN) media - Vancomycin (inhibits gram + organisms), Polymyxin (inhibits gram - organisms except Neisseria), and Nystatin (inhibits fungi);
“to connect to Neisseria, please use your VPN client”
B. pertussis
Bordet-Gengou (potato) agar (Bordet for Bordetella)
C. diphtheriae
Tellurite agar, Loffler medium
M. tuberculosis
Lowenstein-Jensen agar
M. pneumoniae
Eaton agar, requires cholesterol
Lactose-fermenting enterics
Pink colonies on MacConkey agar (fermentation produces acid, turning colony pink); E. coli is also grown on eosin-methylene blue (EMB) agar as colonies with green metallic sheen
Legionella
Charcoal yeast extract agar buffered with cysteine and iron
Fungi
Sabouraud agar.
“Sab’s a fun guy!”