Culture Requirements Flashcards

1
Q

H. influenzae

A

Chocolate agar with factors V (NAD+) and X (hematin)

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2
Q

N. gonorrhoeae, N. meningitidis

A

Thayer-Martin (or VPN) media - Vancomycin (inhibits gram + organisms), Polymyxin (inhibits gram - organisms except Neisseria), and Nystatin (inhibits fungi);
“to connect to Neisseria, please use your VPN client”

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3
Q

B. pertussis

A

Bordet-Gengou (potato) agar (Bordet for Bordetella)

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4
Q

C. diphtheriae

A

Tellurite agar, Loffler medium

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5
Q

M. tuberculosis

A

Lowenstein-Jensen agar

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6
Q

M. pneumoniae

A

Eaton agar, requires cholesterol

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7
Q

Lactose-fermenting enterics

A

Pink colonies on MacConkey agar (fermentation produces acid, turning colony pink); E. coli is also grown on eosin-methylene blue (EMB) agar as colonies with green metallic sheen

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8
Q

Legionella

A

Charcoal yeast extract agar buffered with cysteine and iron

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9
Q

Fungi

A

Sabouraud agar.

“Sab’s a fun guy!”

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