Stains

Question 1

Hematoxylin and eosin

Answer 1

• Hematoxylin detects nucleic acids within nuclei
• It stains blue

Question 2

Eosin

Answer 2

• detects cytoplasmic organelles
• It stains pink

Question 3

Luxol fat blue

Answer 3

• Stains myelin blue ( demyelination causes loss of affinity for the stain)

Question 4

Bodian

Answer 4

• Stains nerve fibers black

Question 5

Movat’s spentachrome

Answer 5

• Stains elastic tissue black

Question 6

Periodic acid-schiff

Answer 6

• Stains basement membrane magenta ( lens capsule, Descemet membrane, Bruch’s membrane)

Question 7

Masson trichrome

Answer 7

• Stains collagen blue or green
• Stains hyaline red
• used for granular dystrophy

Question 8

Congo red

Answer 8

• Stains amyloid orange
• Used for lattice dystrophy

Question 9

Alcian blue

Answer 9

• Stains glycosaminoglycans
• Used for macular dystrophy

Question 10

Wilder’s

Answer 10

• Stains reticulin fibers black

Question 11

Alizarin red

Answer 11

• Stains calcium red/ orange

Question 12

Von kossa

Answer 12

• Stains calcium black
• Used for band keratopathy

Question 13

Prussian blue

Answer 13

• Stains iron blue

Question 14

Mucicarmine

Answer 14

• Stains mucus pink
• Useful stain for mucous secreting adenocarcinoma

Question 15

Grocott hexamine silver

Answer 15

• Stains fungi and acanethamoea black

Question 16

Oil red O

Answer 16

• Stainis lipid red
• E.g. sebaceous gland carcinoma

Question 17

Ziehl neilson

Answer 17

• mycobacteria

Question 18

Gram

Answer 18

Bacteria and fung

Question 19

Giemasa

Answer 19

Acanthamoeba, fungi, cytology and intranuclear inclusion bodies

Question 20

Acid-fast`

Answer 20

Mycobacterium, nocardia

Question 21

Periodic acid Schiff

Answer 21

Fungi

Question 22

Gomori methenamine silver

Answer 22

Fungi

Question 23

Calcofluor white

Answer 23

Fungi and acanthoamoea

Question 24

KOH

Answer 24

fungi

Question 25

blood agar

Answer 25

Bacteria (atypical mycobacterium)

Question 26

Bood agar in carbon dioxide

Answer 26

Moraxella

Question 27

Chocolate age

Answer 27

Haemophillis and Neisseria

Question 28

Thioglycate

Answer 28

Anaerobes

Question 29

Sabourauds

Answer 29

Fungi

Question 30

Lowenstain-jensen

Answer 30

Mycobacterium tuberculosis, nocardia

Question 31

Loeffler’s

Answer 31

Corynebacteria

Question 32

Non-nutrient agar with e.coli

Answer 32

Acaenthaemoeba

Question 33

Corneal stromal dystrophies

Answer 33

Marilyn Monroe Always Gets Her Men in LA City (MMA; GHM; LAC)

Question 34

• Macular dystrophy

Answer 34

Mucopolysaccharide, Alcian Blue ( M,M,A)

Question 35

• Granular dystrophy

Answer 35

Hyaline, Mason Trichome ( G,H,M)

Question 36

• Lattice dystrophy

Answer 36

Amyloid, Congo Red ( L,A, C)