Stains Flashcards

1
Q

Hematoxylin and eosin

A
  • Hematoxylin detects nucleic acids within nuclei
  • It stains blue
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2
Q

Eosin

A
  • detects cytoplasmic organelles
  • It stains pink
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3
Q

Luxol fat blue

A
  • Stains myelin blue ( demyelination causes loss of affinity for the stain)
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4
Q

Bodian

A
  • Stains nerve fibers black
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5
Q

Movat’s spentachrome

A
  • Stains elastic tissue black
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6
Q

Periodic acid-schiff

A
  • Stains basement membrane magenta ( lens capsule, Descemet membrane, Bruch’s membrane)
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7
Q

Masson trichrome

A
  • Stains collagen blue or green
  • Stains hyaline red
  • used for granular dystrophy
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8
Q

Congo red

A
  • Stains amyloid orange
  • Used for lattice dystrophy
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9
Q

Alcian blue

A
  • Stains glycosaminoglycans
  • Used for macular dystrophy
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10
Q

Wilder’s

A
  • Stains reticulin fibers black
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11
Q

Alizarin red

A
  • Stains calcium red/ orange
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12
Q

Von kossa

A
  • Stains calcium black
  • Used for band keratopathy
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13
Q

Prussian blue

A
  • Stains iron blue
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14
Q

Mucicarmine

A
  • Stains mucus pink
  • Useful stain for mucous secreting adenocarcinoma
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15
Q

Grocott hexamine silver

A
  • Stains fungi and acanethamoea black
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16
Q

Oil red O

A
  • Stainis lipid red
  • E.g. sebaceous gland carcinoma
17
Q

Ziehl neilson

A
  • mycobacteria
18
Q

Gram

A

Bacteria and fung

19
Q

Giemasa

A

Acanthamoeba, fungi, cytology and intranuclear inclusion bodies

20
Q

Acid-fast`

A

Mycobacterium, nocardia

21
Q

Periodic acid Schiff

A

Fungi

22
Q

Gomori methenamine silver

A

Fungi

23
Q

Calcofluor white

A

Fungi and acanthoamoea

24
Q

KOH

A

fungi

25
Q

blood agar

A

Bacteria (atypical mycobacterium)

26
Q

Bood agar in carbon dioxide

A

Moraxella

27
Q

Chocolate age

A

Haemophillis and Neisseria

28
Q

Thioglycate

A

Anaerobes

29
Q

Sabourauds

A

Fungi

30
Q

Lowenstain-jensen

A

Mycobacterium tuberculosis, nocardia

31
Q

Loeffler’s

A

Corynebacteria

32
Q

Non-nutrient agar with e.coli

A

Acaenthaemoeba

33
Q

Corneal stromal dystrophies

A

Marilyn Monroe Always Gets Her Men in LA City (MMA; GHM; LAC)

34
Q
  • Macular dystrophy
A

Mucopolysaccharide, Alcian Blue ( M,M,A)

35
Q
  • Granular dystrophy
A

Hyaline, Mason Trichome ( G,H,M)

36
Q
  • Lattice dystrophy
A

Amyloid, Congo Red ( L,A, C)