Staining Part 3 Flashcards
examination of blood to detect blood-borne
parasites like malaria.
romanowsky stain
forms an orange-red lake with calcium at a pH of 4.2. It works best with small amounts of calcium
alizarin red s
Nuclei are deep red; cytoplasm is a pale red.
azocarmine
used for staining hemoglobin
benzidine
used as a chromatin stain for fresh materials in smear preparations
carmine
used for staining blood to differentiate leukocytes
giemsa
oldest of all stains, originally used for microscopic study of starch granules.
iodine
brown solution that turns black in the presence of starches
lugol’s
lipophilic stain; it will accumulate in lipid globules inside cells. can be used with living cells. It fluoresces strongly when
partitioned into lipids, but practically not at all in aqueous solution.
nile red
dye that is more soluble in fat than in water or alcohols, hence it is used as a stain for neutral lipids.
oil red o
all-around useful stain for many
things. It stains glycogen, mucin, mucoprotein, glycoprotein, basement
membranes, capsules, and blood vessels as well as fungi and intracellular
carbohydrates such as glycogen in hepatocytes
pas
common negative stain for viruses,
nerves, polysaccharides,
ptah
contrast stain to acid fuchsin
picric acid
stain blood and glandular tissues.
rhodamine b
a nuclear stain. It produces red nuclei, and is
used primarily as a counterstain. may also be used to give a yellow
color to collagen.
safranin
