Staining Part 2

Question 1

This staining method stains the free fatty acids dark green.

Answer 1

Copper rubeanic acid

Question 2

A smear preparation that is useful for the assessment of cellularity, megakaryocyte
number, focal diseases and fibrotic marrows.

Answer 2

squash smear

Question 3

If tuberculosis or fungal infection is suspected, the fixed sample should be placed in
formalin for how long?

Answer 3

48h

Question 4

It stain the bile pigments green and collagen, muscle and bone pale pink.

Answer 4

giemsa

Question 5

The following are the different dyes used in fluorescent staining for DNA and RNA,
EXCEPT:
rhodamine, fluorochromes, acriflavine, acridine red

Answer 5

acridine red

Question 6

This method is used for nuclear sex determination and for general morphological studies.

Answer 6

feulgen

Question 7

Which of the following statements best describes Sudan Black B:

Answer 7

most sensitive

Question 8

It is useful for the assessment of overall marrow architecture and cellularity and provide
greater sensitivity for the assessment of focal lesions and patchy infiltrates.

Answer 8

trephine

Question 9

The following are techniques in staining nucleic acids, EXCEPT:
feulgen, alcian blue, gallocyanin, methyl green

Answer 9

alcian blue

Question 10

It is used to remove nucleic acid that may mask the
basic group of protamines and histones.

Answer 10

trichloroacetic acid

Question 11

It can be demonstrated by selective staining with small or large-molecule dyes using the
Trichrome method

Answer 11

fibrous proteins

Question 12

It can serve as a sensitive vital stain for the detection of cytoplasmic lipid droplets

Answer 12

nile red

Question 13

It is the gold standard for histologic interpretation of lymph node specimen.

Answer 13

h&e

Question 14

In Feulgen’s method, it is used to remove the excess Schiff’s reagent from the section,
while preventing its oxidation back to basic fuchsin

Answer 14

bisulfite solution

Question 15

A staining method to identify plasma cells, mast cells, lymphoid cells, eosinophils, and for
distinguishing between myeloblasts and proerythroblasts.

Answer 15

giemsa

Question 16

This method can give the DNA a green fluorescence while RNA is stained brick to
orange-red.

Answer 16

fluorescent staining

Question 17

It is the most commonly used fluorochrome to demonstrate DNA and RNA in fresh or
fixed tissues, combining with nucleic acids in cells by salt linkages and cohesion.

Answer 17

acridine orange

Question 18

the following are advantages of microwave ovens, except:

greater profitability, increased efficiency, temperatures maintained at 75-80 C, shorter processing

Answer 18

temp

Question 19

Bone marrow must be fixed with Zenker’s fluid and acetic acid for how long before
processing?

Answer 19

14-24

Question 20

A staining method to demonstrate both DNA and RNA in nucleic acids.

Answer 20

gallocyanin

Question 21

A temperature that can speed up fluid penetration and tissue processing in general.

Answer 21

37-45

Question 22

A dye that emits light or visible radiation energy when excited by the light of shorter
wavelength, either visible or ultraviolet.

Answer 22

fluorescent

Question 23

The preferred staining method for bone marrow aspirate smears

Answer 23

wright

Question 24

The preferred staining method for bone marrow aspirate smears

Answer 24

myeloperoxidase

Question 25

The following are the ways to sharpen nuclear staining, EXCEPt

Answer 25

dehydrate

Question 26

The following are the ways to sharpen nuclear staining, EXCEPt

Answer 26

dehydrate

Question 27

It is the technique that is widely used to separate biological macromolecules, usually
proteins or nucleic acids, according to their electrophoretic mobility.

Answer 27

polyacrylamide

Question 28

It is 60-80% faster than conventional processing
time with an hourly throughput of up to 120 specimens.

Answer 28

Microwave processing

Question 29

It uses the basic dyes to produce a differential staining reaction for DNA and RNA.

Answer 29

Methyl green pyronin

Question 30

The staining method of choice in bone marrow aspirate samples with cytologic dysplasia,
the morphologic hallmark of myelodysplastic syndromes.

Answer 30

may-grunwald

Question 31

A factor that increases the flow of fresh fluids in and around the tissues.

Answer 31

agitation

Question 32

It provides a means of detecting single copies of
nucleic acid sequences in cellular preparations.

Answer 32

pcr