Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Parasitology (lab) > Staining Fecal Smear > Flashcards

Staining Fecal Smear Flashcards

1
Q

Why do we need to stain fecal smears?

A

For the identification of protozoans and sharp definition of the morphologic details of the parasite

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Noted for creating fine morphologic detail and stains intestinal protozoa (both trophozoites and cysts) with great detail

A

Iron Hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

It is a modification of Gomori stain

A

Wheatley’s Trichome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

It is a routine part of the ova and parasite examination for the
identification of Cryptosporidium, Isospora, and Cyclospora

A

Modified Acid Fast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

In this stain helminth eggs and
larvae may be obscured by excess stain

A

Iron Hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

In Wheatley’s Trichome, the background material appears ________ in color and protozoa have a ___________ cytoplasm.

A

greenish and blue-green to purple, respectively

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

This is a very good stain for fresh, PVA
or SAF-preserved fecal smears

A

Iron Hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the 3 organisms differentiated in Modified Acid Fast?

A

Cryptosporidium, Isospora, and Cyclospora

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

This is a very useful stain for fresh and PVA-preserved fecal smear but not with SAF-preserved smears

A

Wheatley’s Trichome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The Modified Acid Fast retain the carbol fuschin and stain ________ against a _____ background.

A

bright red to purple and blue, respectively

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the 3 permanent stains?

A

Iron Hematoxylin, Wheatley’s Trichome, Modified Acid Fast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

In preparing a fecal smear, how many minutes do you fix the fresh stool sample in PVA?

A

30 minutes or overnight

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

In preparing a fecal smear, how many minutes do you fix the fresh stool sample in PVA?

A

30 minutes or overnight

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

In fecal smear preparation, in what motion do you spread the preserved stool?

A

Rolling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

At what temperature do you allow the fecal smear to dry?

A

37°C incubator or overnight at room
temperature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

TRUE OR FALSE: In staining with Iron Hematoxylin, you place the slide in 85% ethanol for 15 minutes.

A

FALSE. Because it should be at 70% ethanol for 5 minutes.

16
Q

TRUE OR FALSE: In staining with Iron Hematoxylin, you wash the slide with running distilled water (constant stream of water into the container) for 10 minutes.

A

FALSE. Because you should wash the slide with running tap water not distilled.

17
Q

How many minutes should you place the slide in iron hematoxylin working solution?

A

4 to 5 minutes

18
Q

In staining with Iron Hematoxylin, what do you add to the stained area before covering the slide with a coverslip?

A

Mountant

19
Q

In what objective do you examine the iron hematoxylin-stained smear microscopically?

A

oil immersion objective

20
Q

Before reporting a negative result in an iron hematoxylin-stained smear, how many fields must you examine?

A

200 to 300 fields

21
Q

Staining with Iron Hematoxylin is for the assessment of?

A
  • Trophozoites
  • Cysts
  • Yeast cells
  • Pseudo hyphae
  • Human cells (Macrophages, PMNs, RBCs)
22
Q

This is used for the detection of Cryptosporidium, Cyclospora, and other Coccidian

A

Acid-Fast Stain (Modified Ziehl Neelsen Technique)

23
Q

When it comes to acid-fast stains, how long do you fix the air-dried smear in methanol?

A

2-3 minutes

24
Q

In which staining method do you stain the feces with cold carbol-fuchsin for 5-10 minutes?

A

Acid-fast stain (Modified Ziehl-Neelsen Technique)

24
Q

In which staining method do you stain the feces with cold carbol-fuchsin for 5-10 minutes?

A

Acid-Fast Stain (Modified Ziehl Neelsen Technique)

25
Q

In Acid-Fast Stain, this is added as a decolorizer.

A

acid-alcohol

26
Q

TRUE OR FALSE: In Acid-Fast stain, you counterstain with 0.25% malachite green or methylene blue for 30 seconds.

A

TRUE

27
Q

What can inexperienced microscopist miss easily?

A

Oocysts

28
Q

Oocysts are elongated ellipsoidal in shape, one _____ or two _____appear inside.

A

sporoblast and sporocysts, respectively

29
Q

A stool smear stained in this stain shows the size difference and staining characteristics between cyclospora cayetanensiis and cryptosporidium spp.

A

modified acid-fast stain

Parasitology (lab) (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions