staining Flashcards
examples of simple stains
methylene blue, crystal violet, safranin
gram stain properties
crystal violet
iodine
alcohol
safranin
endospore stain properties
malachite green
heat (mordant)
water (decolorizer)
safranin
steam is used in endospore stain because
force dye through endospore coat
acid fast stain
basic fuchsin and phenol (primary)
steaming (mordant)
acid-alcohol decorizer
methylene blue (counter stain)
negative stain properties
india ink
congo red
eosin
nigrosin
in negative stain, what stain do we use with nigrosin
india ink
capsule stain
congo red (primary)
marneval’s (counter stain)
drain off no water
what does APO indicate
correction for chromatic aberration
what does E indicate of
do not correct for chromatic aberration
what does Plan indicate of
correction for spherical aberration
what does NE indicate of
light gathering capacity
what happen if you over decolorized gram stain for positive
it probably lose the ability to obtain the color