staining Flashcards

1
Q

examples of simple stains

A

methylene blue, crystal violet, safranin

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2
Q

gram stain properties

A

crystal violet
iodine
alcohol
safranin

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3
Q

endospore stain properties

A

malachite green
heat (mordant)
water (decolorizer)
safranin

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4
Q

steam is used in endospore stain because

A

force dye through endospore coat

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5
Q

acid fast stain

A

basic fuchsin and phenol (primary)
steaming (mordant)
acid-alcohol decorizer
methylene blue (counter stain)

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6
Q

negative stain properties

A

india ink
congo red
eosin
nigrosin

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7
Q

in negative stain, what stain do we use with nigrosin

A

india ink

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8
Q

capsule stain

A

congo red (primary)
marneval’s (counter stain)
drain off no water

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9
Q

what does APO indicate

A

correction for chromatic aberration

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10
Q

what does E indicate of

A

do not correct for chromatic aberration

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11
Q

what does Plan indicate of

A

correction for spherical aberration

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12
Q

what does NE indicate of

A

light gathering capacity

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13
Q

what happen if you over decolorized gram stain for positive

A

it probably lose the ability to obtain the color

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14
Q
A
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