STAINING

Question 1

Mixture of picric acid and acid fuchsin for the demonstration of connective tissues, mucin and elastic tissue

Answer 1

Van Gieson’s (Acid fuchsin picric acid)

Question 2

Basic acridine fluorochrome which permits discrimination between dead and living cells, giving GREEN fluorescence for DNA and a RED fluorescence for RNA

Answer 2

Acridine orange

Question 3

For calcium salts and phosphatase activity

Answer 3

Acridine Red 3B

Question 4

Stain acid mucopolysaccharides
More specific for connective tissue and epithelial mucin

Answer 4

Alcian Blue

Question 5

A cytoplasmic stain used for counterstaining of epithelial sections

Answer 5

Aniline blue

Question 6

A plasma stain utilized also for deep staining of acid fast organisms mitochondria, and differentiation of smooth muscles with the use of picric acid

Answer 6

Basic fuchsin [Carbol fuchsin, Coleman’s feulgen, Schiff’s, Mallory’s fuchsin, Aldehyde fuchsin (Gomori’s)]

Question 7

used for staining hemoglobin

Answer 7

Benzidine

Question 8

used for staining diptheria; used for contrast stain for Gram’s technique, acid fast and papanicolau method

Answer 8

Bismarck brown

Question 9

used as a chromatin stain for fresh materials in smear preparations; combined with aluminum chloride to stain glycogen

Answer 9

Carmine

Question 10

A mordanted dye acting as a basic dye and staining acid substances

Answer 10

Mayer’s Carmalum solution

Question 11

Recommended for routine staining of fixed sections

Answer 11

Celestine blue

Question 12

best known as indicator; may be utilized as a stain for axis cylinders in embryos; used for staining elastic tissues, amyloid and myelin (krajian’s method)

Answer 12

Congo Red

Question 13

A nuclear or chromatin stain used for staining amyloid in frozen sections and platelets

Answer 13

Crystal violet

Question 14

mixture of crystal violet, methyl violet, and dextrin

Answer 14

Gentian Violet

Question 15

used for staining blood to differentiate leukocytes

Answer 15

Giemsa

Question 16

used for metallic impregnation, made up of gold chloride and mercuric chloride

Answer 16

Gold sublimate

Question 17

The OLDEST of all stains; stains amyloid, cellulose, starch, carotenes and glycogen

Answer 17

Iodine (Gram’s, Lugol’s)

Question 18

used for demonstrating mitochondria (intravital stain0

Answer 18

Janus Green B

Question 19

Used as a contrast stain for staining ascaris eggs and erythrocytes; used as a bacterial spore stain

Answer 19

Malachite green

Question 20

stains chromatin green in the presence of an acid

Answer 20

Methyl Green

Question 21

Plasma cells, Fresh sputum for malignant cells, evaluation, and differentiation of bacterial organisms; Diptheria diagnosis and nervous tissue vital staining

Answer 21

Methylene Blue

Question 22

used in frozen sections for rapid diagnosis

Answer 22

Loeffler’s Polychrome Methylene Blue

Question 23

coloring nuclei of leukocytes reddish-purple in presence of methylene blue

Answer 23

Methylene violet

Question 24

for observing cell granules and vacuoles of phagocytic cell

Answer 24

Neutral Red

Question 25

substitute for carbol fuchsin in acid-fast staining

Answer 25

Night blue

Question 26

An excellent stain for Elastic fibers; demonstrates the finest and most delicate fiber sin skin (dermatological studies)

Answer 26

Orcein

Question 27

used as a fixative; used to stain fats

Answer 27

Osmium Tetroxide

Question 28

Normally utilized for the manufacture of paints; used as microanatomical contrast stain for demonstration for the circulatory system by injection (intravital stain)

Answer 28

Prussian blue

Question 29

Used with osmic acid to fix and stain blood and glandular tissues

Answer 29

Rhodamine B

Question 30

Used in identification of Spirochetes, reticulum and other fiber stains

Answer 30

Silver Nitrate

Question 31

Recommended for staining of Nissl granules or chromophilic bodies; nuclear stain for fixed tissues; used as a substitute for thionine in fresh tissue sections

Answer 31

Toluidine blue

Question 32

demonstration of neuroglia in frozen sections

Answer 32

Victoria blue