Spring Exam Flashcards
What does DNA stand for?
Deoxtriblonuecleic Acid
Why is DNA an important molecule?
Contains genetic material
What holds DNA base pairs together in a double helix? Why are there bonds significant?
Hydrogen Bonds
They break when DNA is making proteins
Three types of RNA and functions.
mRNA (messenger)
- carries info in DNA to the ribosomes
tRNA (transfer)
- carries amino acids to the ribosomes
rRNA (ribosmomal)
- make up a ribosome
What is a gamete?
sex cells
What are the two types of gametes?
Sperm cell and egg cell
What is a diploid cell and what is a haploid cell?
diploid cell= 2n # of chromosomes
haploid cell= 1n # of chromosomes
how many chromosomes are there in a human diploid and haploid cell?
diploid: 46
haploid: 23
What type of work did Gregor Mendel do and why is he considered the “father of genetics”?
He worked with pea plants and discovered the patterns of inheritance
What does PCR stand for?
Polymerase Chain Reaction
What is the purpose of PCR?
to make multiple copies of DNA molecule
define the steps of PCR process:
- Denaturing
- Annealing
- Extension
Denaturing (95 C)
Double strands come apart at the base pairs
Annealing (50-60 C)
Pimers attach to the target DNA sequence
Extension (72 C)
DNA Polymerase attaches to the primers and copies to the target sequence
Describe three uses for PCR
Forensic
Tissue indetification in violent crimes
Disease Dignosis
Genetic disease genes can be confirmed
Relational
Can be used to detect and genetic relationship between individuals
What is a clone?
an exact genetic copy of biological matter
Define the following types of clones:
- molecular
- reproductive
- therapeutic
Molecular
copies of DNA molecules
Reproductive
copied of organisms from another organism
Therapeutic
cloning stem cells