Spirochete - Myco Viro Flashcards
Partially Acid-fast Bacteria
Nocardia: Aerobic
Gordonia
Rhodococcus
Tsukamurella
Aerial Hyphae in Tap Water Agar
Nocardia
Nocardiopsis
Streptomyces
anaerobic; agent of Lumpy Jaw/Actinomycosis
causes suppurative granulomatous disease
Actinomyces israelli
Colonies of A. israelli
Molar tooth
primary pulmonary infection resembling TB
N. farcinica & N. cyriacigeorgica
actinomycotic mycetoma
N. brasiliensis
Spirochete
Loosely and helically coiled; longest bacteria
Borrelia
mode of transmission of Borrelia
bite of an infected vector (lice and ticks)
Agent of Louse-born relapsing fever (epidemic)
Borrelia recurrentis
Vector: Pediculus humanus(human louse)
Borrelia recurrentis
Agent of Tick-born relapsing fever
Borrelia anserine, toricatae, parkeri
Vector of Borrelia anserine, toricatae, parkeri
Ornithodoros ticks
Agent of Lyme Disease
Borrelia burgdorferi
Vector of Borrelia burgdorferi
Ixodesdammini(deer ticks)
Stage 1 symptom of Lyme disease
Bull’s eye rash (erythema chronicummigrans)
Stage 2 symptom of Lyme disease
dissemination to blood
Stage 3 symptom of Lyme disease
arthritis, neurological abnormalities, skin lesions
Culture media for Borrelia burgdorferi
Barber Stoenner Kelly at 33C for 6 weeks
tightly coiled spirochete
Treponema
Lab diagnosis for Treponema
Darkfield microscopy - graceful/corkscrew motility
Levaditi silver impregnation
Screening tests for Treponema in serology
Rapid Plasma Reagin (RPR)
Venereal Disease Research Laboratory (VDRL)
Confirmatory tests for Treponema in serology
Fluorescent Treponemal Antibody Absorption (FTA-ABS),
Treponema pallidum Haemagglutination (TPHA),
Microhemagglutination Assay for Treponema pallidum Antibodies (MHA-TP),
Hemagglutination Treponemal Test for Syphilis (HATTS)
Primary Syphilis symptom and specimen of choice
Hard chancre: firm and painless
swab/aspirate; diagnosis using darkfield microscopy
Secondary Syphilis symptom and specimen of choice
condylomata lata (wart-like lesions in genital area or mouth)
serum; (RPR/VDRL, FTA-ABS)
Tertiary Syphilis symptom and specimen of choice
Gummas (granulomatous lesions)
Neurosyphilis (Tabes dorsalis –degeneration of lower spinal cord)
syphilitic cardiovascular lesions
CSF
Hutchinson’s triad (notched teeth, keratitis, nerve deafness)
Congenital syphilis
T. pallidum subsp. pertenue
chronic nonvenereal disease of skin and bones
Yaws/Framboise
T. pallidum subsp. endemicum
lesions in oral cavity/mucosa, skin boes, nasopharynx
Bejel
T. pallidum subsp. endemicum
ulcerative skin disease
Pinta
T. pallidum subsp. carateum
spirochete tightly coiled with 1 or 2 hooks
Leptospira
specimen of choice for 1st week diagnosis of Leptospira
blood, CSF, tissue
specimen of choice for 2nd week diagnosis of Leptospira
urine
Culture method for Leptospira
EMJH (Ellinghausen-McCullough-Johnson-Harris)/Stuart
Fletcher’s
Screening Test for Leptospira
Macroscopic Agglutination Test - (+): agglutination
Gold standard for Leptospira
Microscopic Agglutination Test - (+): agglutination under dark field
cause of leptospirosis (zoonotic infection)
Leptospira interrogans
most common characteristic physical finding in Leptospira interrogans
conjunctival suffusion
severe form of leptospirosis; systemic disease with intravascular disease, renal and hepatic failure
Weil’s disease
Gram-positive obligate intracellular parasite
Chlamydia
of serotypes in C. trachomatis
20
of serotypes in C. pneumoniae
1
of serotypes in C. psittaci
10
agent of psittacosis/ornithosis (parrot fever)
Chlamydophila psittaci
TWAR strain (Taiwan Acute Respiratory)-3rd spp. of Chlamydia
Chlamydophila pneumoniae
TRIC agent (trachoma, inclusion conjunctivitis)
number 1 cause of non-gonococcal urethritis
C. trachomatis
Gram-negative obligate intracellular parasite
Rickettsia
Rocky Mountain Spotted Fever
R. rickettsii (ticks)
Rickettsial pox
R. akari (mites)
Australian/Queensland tick typhus
R. australis (ticks)
Boutonneuse fever, Mediterranean and Israeli spotted fever, Indian tick typhus, Kenyan tick typhus
R. conorii (ticks)
Epidemic typhus
R. prowazekii (Lice)
Sporadic typhus
R. prowazekii (Flying squirrels)
Brill-Zinsser disease
R. prowazekii (Reactivation of latent infection)
Murine typhus
R. typhi (fleas)
Scrub typhus
Orientia tsutsugamushi (mites, chiggers)
Q fever
Coxiella burnetti (ticks, aerosol)
Human monocyte ehrlichiosis
Ehrlichia chaffeensis (ticks)
Human granulocyte anaplasmosis
E. phagocytophila (ticks)
Sennetsu fever
Neorickettsia sennetsu (ticks)
Trench fever
Rochalimea quintana (Lice)
Smallest free-living organisms
Mycoplasma
Eaton’s agent
primary atypical pneumonia/walking pneumonia
M. pneumoniae
Confirmatory for M. pneumoniae
Hemadsorption test
large fried egg colonies
M. hominis
agent of salpingitis and post partal fever
non-gonococcal urethritis in males, PID
M. hominis
non-gonococcal urethritis in males, PID, amnionitis
U. urealyticum
small fried egg colonies with dark brown clumps
U. urealyticum
MOT of Rochalimea quintana
Human body louse
MOT of B. bacilliformis
Sandflies
MOT of B. henselae
Domestic cat by bite/scratches; cat fleas
MOT of B. clarridgeia
Domestic cat by bite/scratches
MOT of B. elizabethiae
Fleas (from rat)
Endocarditis
Koch’s bacillus
Mycobacteria tuberculosis
Virulence factor of M. tuberculosis
Cord factor (produces serpentine cords on agar)
Resistance of M. tuberculosis
Highly resistant to drying
Resistant to chemical disinfection; requires 24-hour exposure to 5% phenol
M. tuberculosis remains in putrifying sputum for how many weeks?
weeks 8-10 days or dried sputum for weeks
M. tuberculosis remains infectious in drops of dried sputum in the air for how many months?
6-8 months
M. tuberculosis is easily killed by?
moist heat, boiling for 10 mins, pasteurization
Treatment (6 months) for M. tuberculosis
Rifampicin, Isoniazid, Pyrazinamide, Ethambutol
Kinyoun
stain preferred for tissues
Also used for M. leprae, Nocardia;
Uses hematoxylin as counterstain
Fite-Faraco’s
(+): yellow-fluorescent organisms on black background
Auramine-Rhodamine
Stain for colorblind; black
Spengler’s
Stain
MTB: red
M. smegmatis: blue
Pappenheimer’s
Stain
MTB: blue
M. leprae: red
Baungarten’s
Culture (confirmatory) - cauliflower colonies
Examples of egg-based (with malachite green)
Lowenstein-Jensen
Petragnani: for contaminated specimens
American Thoracic Society medium
Dorset egg medium
Culture (confirmatory) -cauliflower colonies
Examples of agar based
Duboi’s Oleic Acid Albumin medium
Middlebrook 7H10, 7H11 –clear media used for AST
Mitchinson’s medium
Culture (confirmatory) -cauliflower colonies
Examples of liquid media
Septi-Check
Bactec 12B
Middlebrook 7H9/7H12
Dubos Tween Albumin
Most common cause of TB in Africa
M. africanum
Most common mycobacterium in AIDS patients
Diarrhea
M. avium complex (MAC)
TB in animals;
Bacillus Calmette & Guerin (BCG) vaccine
M. bovis
granulomatous lesions seen on chest X-rays
Tubercles
calcified lesions
Ghon complexes
Sputum specimen for Mycobacteria
2 specimens in 1 day
RUNYOUN CLASSICATION OF MOTT
GROUP 1: PHOTOCHROMOGENS
M. kansasii
M. marinum
M. simiae
M. asiaticum
RUNYOUN CLASSICATION OF MOTT
GROUP 2: SCOTOCHROMOGENS
M. scrofulaceum M. szulgai M. xenopi M. gordonae M. flavescens M. thermoresistible
RUNYOUN CLASSICATION OF MOTT
GROUP 3: NONPHOTOCHROMOGENS
M. avium M. avium-intracellularecomplex (MAC) M. malmoense M. ulcerans M. haemophilum M. terrae-triviale
RUNYOUN CLASSICATION OF MOTT
GROUP 4: RAPID GROWERS
M. fortuitum
M. chelonae
M. phlei
M. smegmatis