Spill Response

Question 1

What are the probable causes of biological spill?

Answer 1

Human

Mechanical

Natural

Question 2

Differentiate Major and Minor biological spill.

Answer 2

Major: Any biological spill occurred outside the BSC (regardless the quantity) in BSL3

Minor: Any biological spill occurred within the BSC.

Question 3

Where should spill kit be placed inside the laboratory?

Answer 3

It should be place somewhere that is readily available and accesible.

Question 4

When there is a biological spill and the agent is unkown, what level of risk grade should be assumed?

Answer 4

Risk grade 4

Question 5

During a biological spill, PPE must be selected based on what?

Answer 5

Based of risk assessment

Question 6

What are the contents of biological spill kit?

Answer 6

1. Plastic hard-walled container to hold the kit contents.
2. Sharps container.
3. Reusable items container.
4. PPE (minimum) which includes:
   a. 2 pairs of goggles/face shield
   b. 2 pairs of nitrile gloves
   c. 2 face mask and N95 respirator
   d. 2 pairs plastic, vinyl or rubber shoe covers
   e. 2 pieces head cover
   f. 2 disposable gowns and coveralls
5. Absorption materials
   a. towels/paper towels
   b. commercial spill pads/spill pillows
6. Cleanup tools and materials
   a. small disposable broom
   b. biohazard waste bags
   c. plastic ties/rubber bands
   d. forceps and tongs
   e. concentrated bleach with bottled water for dilution
   f. 70% alcohol

Question 7

What are the general guidelines for ALL biological spills?

Answer 7

1. Report all spills to the Biosafety officer & supervisor
2. Perform hand hygiene before and after clean-up
3. Don appropriate PPE before clean-up.
4. Use freshly prepared 10% household bleach solution; allow at least 20-30 mins contact time.
5. Dispose off clean-up materials as biohazard waste and place on a biohazard bag. Then autoclave

Question 8

Give the steps for a proper biological spill response.

Answer 8

1. Do not panic
2. Inform/Alert the people
3. Remove contaminated clothing and wash affected area with water and soap (if applicable)
4. Perform hand hygiene and leave the area of spill
5. Close the area, post a Do Not Enter sign

Question 9

In a biological spill clean-up, if the biological agent is a non-viscous, the contact time between the agent and the disinfectant should be?

Answer 9

15-20 minutes

Question 10

In a biological spill clean-up, if the biological agent is a viscous spill, the contact time between the agent and the disinfectant should be?

Answer 10

30 minutes disinfection