SPERM MORPHOLOGY Flashcards

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1
Q

Which part of the sperm cell contains the mitochondrial sheath?
A) Head
B) Neck/Middle Piece
C) Tail
D) Flagella

A

Answer: B) Neck/Middle Piece

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2
Q

What is the length of the tail of a typical sperm cell?
A) 35um
B) 45um
C) 55um
D) 65um

A

B) 45um

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3
Q

hat percentage of the sperm head should the acrosomal cap occupy?
A) 1/4
B) 1/2
C) 2/3
D) 3/4

A

Answer: C) 2/3

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4
Q

Which part of the sperm cell contains the mitochondrial sheath?
A) Head
B) Neck/Middle Piece
C) Tail
D) Flagella

A

Answer: B) Neck/Middle Piece

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5
Q

What is the primary function of the tail of a sperm cell?
A) Attaches the tail to the head
B) Contains the acrosomal cap
C) Provides energy for flagellar motion
D) Propels the sperm cell forward

A

Answer: D) Propels the sperm cell forward

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6
Q

Which part of the sperm provides energy for flagellar motion?
A. Acrosomal cap
B. Mitochondrial sheath
C. Tail
D. Head

A

B. Mitochondrial sheath

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7
Q

What staining method is commonly used for evaluating sperm cells under OIO examination?
A) Giemsa stain
B) Papanicolaou stain
C) Wright’s stain
D) All of the above

A

Answer: D) All of the above

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8
Q

Which stain is considered the stain of choice for evaluating sperm cells under OIO examination?
A) Giemsa stain
B) Papanicolaou stain
C) Wright’s stain
D) None of the above

A

Answer: B) Papanicolaou stain

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9
Q

hat is the normal value for sperm cells under Kruger’s Strict Criteria?
A) >30% normal
B) >14% normal
C) <30% abnormal
D) <14% abnormal

A

Answer: B) >14% normal

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10
Q

Which abnormality is associated with decreased motility and clumping of sperm?
A) Lack of seminal vesicle support medium
B) Male antisperm antibodies
C) Decreased count
D) Sperm agglutination with female serum

A

B) Male antisperm antibodies

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11
Q

What is the primary cause of decreased count in sperm analysis?
A) Lack of seminal vesicle support medium
B) Female antisperm antibodies
C) Viability assessed by Eosin-nigrosin stain
D) Fructose level abnormality

A

A) Lack of seminal vesicle support

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12
Q

Which test is used to detect male antisperm antibodies in sperm analysis?
A) Mixed agglutination and immunobead tests
B) Fructose level
C) Eosin-nigrosin stain
D) Lack of seminal vesicle support medium

A

A) Mixed agglutination and immunobead tests

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13
Q

What test is commonly used to assess viability in sperm analysis?
A) Fructose level
B) Eosin-nigrosin stain
C) Lack of seminal vesicle support medium
D) Mixed agglutination and immunobead tests

A

B) Eosin-nigrosin stain

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14
Q

What color are living sperm cells stained with Eosin-Nigrosin?
A) Red
B) Blue
C) Purple
D) Bluish-white

A

D) Bluish-white

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15
Q

What is the primary indicator of sperm viability in the Bloom’s test?
A) Presence of male antisperm antibodies
B) Percentage of living cells in the sperm sample
C) Fructose concentration in semen
D) Sperm motility

A

B) Percentage of living cells in the sperm sample

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16
Q

What is considered normal under this test?
A) 50% living cells/100 sperm cells
B) 75% living cells/100 sperm cells
C) 90% living cells/100 sperm cells
D) 65% living cells/100 sperm cells

A

Answer: B ) 75% living cells/100 sperm cells

17
Q

How are dead cells identified in this test?
A) Bluish-White in color
B) Green against a White Background
C) Red against a Purple Background
D) Black against a Yellow Background

A

Answer: C) Red against a Purple Background

18
Q

Which test involves boiling a semen sample with a reagent to produce an orange-red color as a screening test for fructose?
A) Resorcinol Test
B) Spectrophotometric Test
C) Microscopic examination
D) Immunobead test

A

A) Resorcinol Test

19
Q

What is the normal value for fructose level in semen as quantitated by spectrophotometric testing?
A) ≥10 umol/ejaculate
B) ≥13 umol/ejaculate
C) ≥15 umol/ejaculate
D) ≥20 umol/ejaculate

A

Answer: B) ≥13 umol/ejaculate

20
Q

Why is freezing semen samples sometimes recommended before Fructose Testing?
A) To increase fructose levels
B) To prevent fructolysis
C) To enhance sperm motility
D) To decrease sperm count

A

B) To prevent fructolysis

21
Q

Which chemical value indicates a disorder of the epididymis when it’s less than the normal value?
A) Fructose
B) Neutral α-glucosidase
C) Zinc
D) Citric acid

A

B) Neutral α-glucosidase

22
Q

Which chemical value indicates a lack of prostatic fluid when it’s less than the normal value?
A) Acid phosphatase
B) Zinc
C) Citric acid
D) Neutral α-glucosidase

A

A) Acid phosphatase
B) Zinc
C) Citric acid
D) Neutral α-glucosidase

23
Q

How do antisperm antibodies (ASA) affect the blood-testes barrier?
A) They strengthen the barrier
B) They have no effect on the barrier
C) They destroy the barrier
D) They block sperm production

A

C) They destroy the barrier

24
Q

What is the primary method used to detect IgG antibodies associated with antisperm antibodies (ASA)?
A) Immunobead test
B) Mixed agglutination reaction (MAR)
C) Fructose test
D) Spectrophotometric test

A

B) Mixed agglutination reaction (MAR)

25
Q

In the Mixed Agglutination Reaction (MAR), what percentage of agglutination is considered positive for IgG antibodies?
A) >50% agglutination
B) >20% agglutination
C) >75% agglutination
D) >90% agglutination

A

> 50% agglutination
20% agglutination

26
Q

What percentage of sperm with bound immunobeads is considered positive in the Immunobead test for antisperm antibodies (ASA)?

A) ≥ 5% sperm with bound immunobead
B) ≥ 10% sperm with bound immunobead
C) ≥ 15% sperm with bound immunobead
D) ≥ 20% sperm with bound immunobead

A

D) ≥ 20% sperm with bound immunobead

27
Q

Which test involves incubating sperm cells with species-nonspecific eggs to observe penetration microscopically?
A) Hamster Egg Penetration
B) Cervical Mucus Penetration
C) Hypo-osmotic Swelling
D) In-Vitro Acrosome Reaction

A

A) Hamster Egg Penetration

28
Q

What is observed in the Cervical Mucus Penetration test?
A) Membrane integrity
B) Sperm motility
C) Sperm penetration ability of cervical mucus
D) Acrosome reaction

A

C) Sperm penetration ability of cervical mucus

29
Q

What does the Hypo-osmotic Swelling test evaluate?
A) Acrosome integrity
B) Sperm viability and membrane integrity
C) Sperm penetration ability
D) Acrosome reaction

A

B) Sperm viability and membrane integrity

30
Q

What is the Sims-Huhmer Test primarily used for?
A) Assessing sperm motility
B) Evaluating sperm penetration ability
C) Determining cervical mucus quality
D) Measuring acrosome integrity

A

C) Determining cervical mucus quality

31
Q

What substance does the Florence Test primarily detect?
A) Choline
B) Spermine
C) Potassium iodide
D) Iodine crystals

A

A) Choline

32
Q

Which reagents are used in the Barbiero’s Test to detect Spermine?
A) Potassium iodide and iodine crystals
B) Picric acid and trichloroacetic acid
C) Florence reagent and water’s solution
D) Spinbarkeit reagent

A

B) Picric acid and trichloroacetic acid

33
Q

What does the presence of brown rhombic or needle-like crystals indicate in the Florence Test?
A) Presence of choline
B) Absence of choline
C) Presence of spermine
D) Absence of spermine

A

A) Presence of choline

34
Q

What property does the Spinbarkeit Test determine?
A) Tenacity of mucus
B) Presence of choline
C) Presence of spermine
D) Semen viscosity

A

A) Tenacity of mucus

35
Q

What is the Barbiero’s Test primarily used to detect?
A) Choline
B) Spermine
C) Semen viscosity
D) Mucus tenacity

A

B) Spermine

36
Q

What color is indicative of a positive result in Barbiero’s Test?
A) Brown
B) Yellow
C) Red
D) Blue

A

B) Yellow

37
Q

BONUS CARD:

BASIC o HINDI BASIC

A

haha ganito kasi, Gusto ko lang sabihin na may pagtingin ako saiyo at nagugustuhan kita, at ito ay hindi sa basic na paraan. Kung mamarapatin mo, gusto ko pa ring makilala ka nang masinsinan.