SEMEN ANALYSIS

Question 1

Where does the sperm mature and develop flagella?

Answer 1

Epididymis

Question 2

Which gland produces most of the fluid present in semen?

Answer 2

Seminal vesicle

Question 3

What is the function of Sertoli cells in the testis?

Answer 3

Provide support and nutrients for germ cells

Question 4

Which component is responsible for the gray appearance of semen?

Answer 4

Flavin

Question 5

Where is the prostate gland located?

Answer 5

Below the bladder

Question 6

What is the primary method of collection for semen analysis to prevent contamination?

Answer 6

Self-production or masturbation

Question 7

What does prolonged liquefaction of semen indicate?

Answer 7

Deficiency in prostatic enzyme

Question 8

What is the normal pH range of semen?

Answer 8

7.2 - 8.0

Question 9

Which condition is characterized by a decrease in the number of sperm cells and few motile cells?

Answer 9

Oligospermia

Question 10

What is the primary factor affecting the penetrating ability of sperm cells through the cervical mucus barrier?

Answer 10

Sperm motility

Question 11

What is the normal volume range of semen?

Answer 11

2-5 mL

Question 12

Which method is used to assess sperm motility based on sperm velocity and trajectory?

Answer 12

Computer-assisted semen analysis

Question 13

Which diluting fluid is traditionally used to immobilize and preserve sperm cells?

Answer 13

2.1% formalin
1.5% sodium bicarbonate

Question 14

What does an increased alkaline pH in semen indicate?

Answer 14

Infection within the reproductive tract

Question 15

Which condition is characterized by the complete absence of spermatozoa?

Answer 15

Azospermia

Question 16

What is the function of the bulbourethral gland (Cowper’s gland)?

Answer 16

Produces alkaline mucus to neutralize acidity in the vagina

Question 17

Which part of the male reproductive system surrounds the urethra and produces 20% - 30% of the fluid in semen?

A. Bulbourethral Gland
B. Prostate Gland
C. Vas Deferens
D. Leydig Cells

Answer 17

B. Prostate Gland

Question 18

What is the primary function of the prostate gland?

A. Produces testosterone
B. Aids in sperm production
C. Produces alkaline mucus
D. Produces fluid for semen

Answer 18

D. Produces fluid for semen

Question 19

Which gland contributes about 5% of the fluid volume in semen and forms thick alkaline mucus?

A. Bulbourethral Gland
B. Prostate Gland
C. Vas Deferens
D. Leydig Cells

Answer 19

A. Bulbourethral Gland

Question 20

What cells are responsible for producing testosterone in the male reproductive system?
A. Bulbourethral Gland
B. Prostate Gland
C. Vas Deferens
D. Leydig Cells

Answer 20

D. Leydig Cells

Question 21

COMPOSITION
Spermatozoa:
Seminal fluid:
Prostate Fluid:
Alkaline mucus:

Answer 21

Spermatozoa: 5%
Seminal fluid: 60-70%
Prostate Fluid: 20-30%
Alkaline mucus: 5%

Question 22

Produced most of the fluid present in the
semen 60%-70%; transport medium of the
sperm

Answer 22

SEMINAL VESICLE

Question 23

metabolized by spermatozoa
for the energy of the flagella to propel
them

a. fructose
b. flavin

Answer 23

Fructose

Question 24

How should specimens be preserved for artificial insemination?

A) At room temperature
B) Preserved in frozen state at −85°C and stored for one year
C) In a refrigerator
D) No preservation needed

Answer 24

B) Preserved in frozen state at −85°C and stored for one year

Question 25

What color indicates urine contamination in semen?
A) Red
B) Green
C) Yellow
D) Blue

Answer 25

C) Yellow

Question 26

What does an increased pH level in semen indicate?
A) False decreased sperm count
B) Increased fertility
C) Normal condition
D) None of the above

Answer 26

A) False decreased sperm count

Question 27

What is not a reliable method of collection because the first portion of ejaculate may be lost?

A) Self-production or masturbation
B) Condom collection
C) Coitus interruptus
D) Vaginal vault aspiration

Answer 27

C) Coitus interruptus

Question 28

This may indicate a
possible infection within the reproductive
tract.

Increased alkaline pH
Decreased alkaline pH

Answer 28

Increased alkaline pH

Question 29

Sperm cells are immobilized by heating.

Makler
Neubauer hemocytometer

Answer 29

Makler

Question 29

Associated with
increased prostatic fluid, ejaculatory duct
obstruction, and poorly developed seminal
vesicles.

Increased alkaline pH
Decreased alkaline pH

Answer 29

Decreased alkaline pH

Question 30

Utilizes undiluted specimen.

Makler
Neubauer hemocytometer

Answer 30

Makler

Question 31

Counts should agree within 10%

Makler
Neubauer hemocytometer

Answer 31

Neubauer hemocytometer

Question 32

What does a grade of 4 indicate in the sperm motility grading criteria?
A. No forward progression
B. Rapid, straight motility
C. Slow forward progression, noticeable lateral movement
D. No movement

Answer 32

B. Rapid, straight motility

Question 33

Which WHO grade corresponds to “No forward progression”

A. A
B. B
C. C
D. D

Answer 33

Answer: C

Question 34

In alternative sperm motility grading criteria, what does “IMMOTILITY” mean?
A. Sperm moving linearly or in a large circle
B. Sperm moving with an absence of progression
C. No movement

Answer 34

Answer: C

Question 35

In alternative sperm motility grading criteria, what is described as “SPERM MOVING LINEARLY OR IN A LARGE CIRCLE”?
A) Progressive Motility
B) Nonprogressive Motility
C) Immotility

Answer 35

Answer: A

Question 36

VISCOSITY
- Normal:
- Reporting: 0 (watery) – 4 (gel-like

Answer 36

viscid to highly viscid
0 (watery) – 4 (gel-like)

Question 37

should be frozen if
delay in testing is unavoidable

Artificial insemination
Fructose level Testing

Answer 37

Fructose level Testing

Question 38

inflammation or
infection of the reproductive system that can
lead to infertility

> 1 MILLION WBC per mL

> 1 MILLION spermatids per mL

Answer 38

> 1 MILLION WBC per mL

Question 39

disruption
of spermatogenesis

Answer 39

> 1 MILLION spermatids per mL