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BI > Spectroscopy > Flashcards

Spectroscopy Flashcards

1
Q

spectroscopy definition

A

study of interaction between radiation+ matter as function of wavelength

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2
Q

Examples

A

Infared, circular Dichroism,Raman

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3
Q

Colour wheel

A

complementary colours diametrically opposite

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3
Q

Wavelength max

A

wavelength of light absorbed most strongly

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4
Q

Absorbance equation

A

log 10 ( transmitted light/ incident light )

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5
Q

Beer Lambert Law

A

Aborbance= extinction coefficient x conc x path length

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6
Q

Qualitative Analysis

A

Finding out identity of diff compounds in samples

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7
Q

Quantitative Analysis

A

measuring conc or amount of each compound in sample ( calibration curve)

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8
Q

Peptide bond resonance Structure

A

electrons delocalized over pi bond (Shared)

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9
Q

Torsion (dihedral) angle

A

3 consecutive bonds + defined by angle created between 2 outer bonds

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10
Q

phi angle

A

N - C (alpha)

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11
Q

psi angle

A

C (alpha)- C

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12
Q

diff phi + psi angles =

A

duff secondary Structures

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13
Q

Ramachandran Plot

A

plot diff phi + psi angles + identifies secondary structures

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14
Q

Wavelength max effected by: x3

A

angle of bond, environment, H-Bonding

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15
Q

FTIR Used for

A

Bond which vibrate /twist in response to IR + absorb photons

16
Q

How spectroscopy used in diagnosis

A

changes in metabolic pattern may produce spectroscopic markers = identification

17
Q

Raman Spectrescopy Benefits

A

Non-destructive chemical analysis

18
Q

Raman identifies / uses

A

identify chemical compounds/ functional groups, conformation or complex biomolecules, chemical composition /structure + in vivo measurements (during surgery)

19
Q

Raman measures

A

degree of scatter light

20
Q

Fluorescence Principle

A

Absorption of light at 1 wavelength + emission at longer wavelength

21
Q

Intersystem crossing due to

A

delay of vibrational relaxation

22
Q

Quantum Dot

A

semi - conducting single crystals used as diagnostic/ therapeutic

23
Q

Using Quantum Dots for Carb changes

A

Bind lectin , Lectin binds carb = fluorescence or treatment

BI (12 decks)

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