Microscopy Flashcards

1
Q

Benefits

A

Rapid Results

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2
Q

2 refractive lens

A

converging ()
Diverging ) (

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3
Q

Bright field Principle+ diagnosis

A

stain required = non -viable tissue
Diagnoses cancers + infectious disease e_g Gonorrhoea.

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4
Q

Dark field

A

uses diff refractive indices of cell component = increase contrast
Living tissues e.g Syphilis

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5
Q

phase contrast

A

Enhances natural contrast (no dye)
2 extra components : annular+ phase ring e.g Gout

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6
Q

Fluorescent

A

High contrast + specificity
Quantitative
Live cell imaging compatible
E.S TB

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7
Q

Stokes shift

A

differences between absorption + emission wavelength

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8
Q

Electron

A

Requires fixing
No live cell imaging
High resolution, contrast by post image colouring
Eg DNA transcription

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