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Clinical Biochem > Spectrophotometry > Flashcards

Spectrophotometry Flashcards

1
Q

A ________ is used to provide a source of light at certain energy, _______, and to measure the quantity of light that is absorbed by the sample.

A

Spectrophotometer, wavelength

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2
Q

______ is the amount of light that has been absorbed by the sample; and the relationship between sample concentration and absorbance is _____ up to a certain concentration.

Whereas, ____ is the fraction of incident light that passes completely through the sample; and the relationship between the sample concentration and transmittance is _________

A

Absorbance
Linear (directly proportional)

Transmittance

Exponential (inversely proportional)

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3
Q

A spectrophotometer is designed to detect _________ of light at different __________when the light passes through a solution of some given concentration.

A

Absorbance, wavelength

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4
Q

A spectrophotometer is a method of analyses that involves how _______ interacts with the atoms (or molecules) in a sample of matter.

A

Light

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5
Q

The _______ spectrum consists of electromagnetic radiation whose wavelengths range from 400 nm to nearly 800 nm.

A

Visible

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6
Q

When this light passes through a substance, certain energies (or colours) of the light are _____ while other colour(s) are allowed to pass through or are _______ by the substance.

A

Absorbed

Reflected

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7
Q

A substance appears white if it does not absorb ________

A

Any colour

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8
Q

We see _____ colour when the substance doesn’t absorb any white light.

A

White

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9
Q

We see ______ colour when the substance does absorb all colours except red colour

A

Red

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10
Q

The visible range is from ______ nm.

A

400 - 780

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11
Q

The ultraviolet range is ______ nm.

A

Below 400

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12
Q

The visible light is used to detect _____ substances, whereas the ultra-violet is used for ______ substances.

A

Coloured

Colourless

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13
Q

A ______ is either a prism or a diffraction grating that seperates the white light into all colours of the visible spectrum

A

Monochrometer

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14
Q

A _____ Lamp is used for Vis spectrophotometer

A

Tungstin

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15
Q

A _______ Lamp is used for UV spectrophotometer

A

Deuterium/Hydrogen/Mercury

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16
Q

Conjugated aromatic compounds, such as aromatic amino acids absorb _______ radiation

A

Ultraviolet

17
Q

Compounds that absorb UV radiation are normally measured in cuvettes made of _______

A

Quartz

18
Q

What is the optimum pH of pepsin?

A

2 or 3

19
Q

The enzyme’s theory that state that “Enzyme structure is flexible, not rigid substrate binding induces appropriate conformational changes in the enzyme Increases range of substrate specificity.” is termed: ————————.

A

Induced-fit theory

20
Q

The enzyme’s theory that state that “The shape of the active site is complementary to that of the substrate molecule.” is termed: ————–.

A

Key-Lock theory

21
Q

What is the optimum pH of trypsin?

A

8

22
Q

In non competitive inhibitors, the inhibitor fits into ————– site

A

allosteric

23
Q

In non competitive inhibitors, the inhibitor fits into _______ site

A

Active site

24
Q

The extinction coefficient of a solution containing 1g molecule of solute per 1 liter of solution is termed _______

A

Molar absorptivity or molar extention coefficient

25
Q

The maximum ———is the one at which a compound gives its highest absorbance.

A

Wavelength

26
Q

The slope of a standard curve divided by the cuvette’s path length is equal to _________

A

Molar absorptivity of molar extention coeficient

27
Q

The equation for Beers-Lambert law is _______

A

A = (wierd E boi) x C x L

28
Q

The cuvette with a path length of 0.4 cm gave an absorbance of 0.6. What is the absorbance when the path length is doubled?

A

1.2

29
Q

Best results for unknown concentration is obtained when the absorbances are between ———–

A

0.1 - 1.0

30
Q

The molar absorptivity deviates at concentrations

A

> 0.01 M

31
Q

The enzyme’s uses the process termed “______ “to (i) prevent waste of cell raw materials (substrates), (ii) prevent overproduction of cell products and (iii) prevent wastes of cell energy

A

“switching on/off”

32
Q

The ability of a compound to absorb light is termed _________

A

Molar absorptivity or molar extinction coefficient

33
Q

Conjugated aromatic compounds such as aromatic amino acids are absorbed at which spectra?

A

Ultra violet

34
Q

According to Beers-Lambert law, the relationship between the absorbance and transmittance is ______ proportional

A

Indirectly

35
Q

The relationship between the absorbance and transmittance is __________

A

A = 2 - Log %T

or

A = Log (1/T)

36
Q

—— light is actually a mixture of all the colors of light.

A

White

Clinical Biochem (2 decks)

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