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Chemistry Lecture > Spectrophotometry > Flashcards

Spectrophotometry Flashcards

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1
Q

way of characterizing a compound by the way it interacts with light either by absorbing or emitting

A

spectrophotometry

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2
Q

determined by how its electrons interact with light in the UV, visible and near IR regions

A

absorbance of a compound

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3
Q

break bonds and release electrons

A

x-rays

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4
Q

absorbed by molecules

A

UV and visible

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5
Q

caused molecules to vibrate

A

infrared

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6
Q

allows you to choose a single wavelength

A

monochrometer

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7
Q

used multiple wavelengths and light diffracted after

A

polychrometer

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8
Q

light source of visible spectrum

A

quartz halogen lamp

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9
Q

light source of UV spectrum

A

deuterium lamp

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10
Q

types of monochromators

A

filters, prisms, diffraction gradients

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11
Q

isolates the light of a desired wavelength

A

monochromator

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12
Q

absorption cells

A

cuvets

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13
Q

types of cuvets

A

glass, silica (quartz), plastic

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14
Q

devices that convert light to an electric signal that is proportional to the number of photons striking the photosensitive surface

A

photodetector

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15
Q

types of photodetector

A

barrier layer, photo tube, photomultiplier, photodiode

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16
Q

releases electrons when light strikes, electron moves around and measures change in voltage

A

phototube

17
Q

electron travels plate to plate and doubles electron eventually reaching x10e-

A

photomultiplier tube

18
Q

silicon chip converts light into electrical signal

A

photodiode

19
Q

calibration of a spectrophotometer measures

A

linearity and wavelength

20
Q

how linearity is measured

A

didymium filter

21
Q

how wavelength is measured for calibration

A

holmium oxide filter

22
Q

the absorbance of a solution is directly proportional to the solutions concentration

A

beers law

23
Q

the amount of light absorbed by an analyze at a specific wavelength

A

absorptivity coefficient

24
Q

the absorptivity coefficient expressed interims of moles

A

molar absorptivity coefficient

25
Q

is the amount of light absorbed by the solution in cuvette

A

absorbance

26
Q

is the amount of light that passes through the cuvette

A

transmittance

Chemistry Lecture (4 decks)

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