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Clinical Chemistry > Spectrophotometry > Flashcards

Spectrophotometry Flashcards

0
Q

Most common light source

A

Tingsten light bulb

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1
Q

Oldest yet most commonly used

A

Colorimetry

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2
Q

Parts of a spectrophotometer

A

Lemecpm

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3
Q

Other kinds of light source

A

Mdhxmg

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4
Q

Minimizes unwanted or stray light

A

Entrance slit

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5
Q

Isolates specific or indiv wavelengths

A

Monochromator

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6
Q

Prevents the entrace of scattered light

A

Entrance slit

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7
Q

Wedge shape piece of glass, quartz or nacl

A

Prism

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8
Q

Made by cutting grooves or slit in an aluminized surface

A

Diffraction gratings

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9
Q

Simple, least expensive monochromator

A

Filter

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10
Q

Holds the sample

A

Cuvet

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11
Q

Semi transparent silver films on both sides of a dielectric

A

Filter

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12
Q

Kinds of cuvet

A

Aqbs

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13
Q

Composed of selenium on a plate of iron covered with transparent layer of silver

A

Barrier layer

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14
Q

Contains cathode and anode enclosed in a glass case

A

Phototube

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15
Q

Most common type of photodetector

A

Photomultiplier tube

16
Q

Photodetector that requires no external voltage

A

Barrier layer

17
Q

Gives of electrons when light strikes it

A

Photo tube

18
Q

Measure light in a multitude of wavelengths

A

Photodiode

19
Q

Also known as absorption cell, analytical cell or sample cell

20
Q

Detects less amt of light

A

Photodiode

21
Q

State the beer’s law

A

Concentration of the inknown substance is directly proportional to the absorbed light and inversely proportional to the amt of transmitted light

22
Q

Amt of light absorbed

A

Absorbance

23
Q

Used to assess wavelength accuracy

A

Didymium & holmium oxide filter

24
Q

To assess absorbance accuracy

A

Neutral density filter and dichromate sol.

25
Q

State 5 poisonous vapors

A

Ccl4 chloroform mercury bromide ammonia

26
Q

5 flammable and combustible solvents

A

Acetone ethanol toluene methanol cylene

Clinical Chemistry (6 decks)

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