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Clinical Chemistry > Other Analytical Methods > Flashcards

Other Analytical Methods Flashcards

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0
Q

Excitation of electrons from lower to higher energy

A

Flame emission photometry

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1
Q

Determines the amt of light blocked

A

Turbidetry

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2
Q

Light source of of atomic absorption spectrophotometry

A

Hollow cathode lamp

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3
Q

Determines the amount of scattered light at various angles

A

Nephelometry

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4
Q

Unknown sample is made to react with a known solution in the presence of an indicator

A

Volumetric (titrimetric)

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5
Q

Measures light produced by atoms burned by a flame

A

Flame emission photometry

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6
Q

Standard pH

A

8.6

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7
Q

Chlorine test

A

Schales and schales method

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7
Q

Migration of charged particles in an electric field

A

Electrophoresis

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8
Q

Most common pH buffer

A

Pheno barbital/ veronal/barbital

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10
Q

Calcium test

A

Edta titration method

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11
Q

Used to scan and quantitate electrophoretic patterns

A

Densitometry

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12
Q

PH of tris boric edta

A

8.7

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12
Q

Stain for proteins and enZyme

A

Amino black, ponceau s

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14
Q

Ph of veronal

A

8.6

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15
Q

Stains for fat and lipoproteins

A

Oil red o / sudan black/ fat red 7B

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16
Q

Stain for csf proteins

A

Coomassie blue

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17
Q

Measures the absorbance of the molecules

A

Densitometry

17
Q

Supporting media for isoelectric focusing

A

Agarose gel
Polyacrylamide gel
Cellulose acetate

19
Q

Separates molecules through a pH gradient

A

Isoelectric focusing

20
Q

2 forms of chromatography

A

Planar

Column

21
Q

2 types of column chromatography

A

Gas

Liquid chromatography

22
Q

Separation based on the sign and ionic charge density

A

Ion exchange chromatography

23
Q

Chromatography for volatile substances

A

Gas chromatography

24
Q

Chromatography that uses lower temp and easier to recover samples

A

Liquid chromatography

25
Q

Separates molecules based on the differences in their size and shape

A

Gel/ gel permeation / gel filtration chromatography

26
Q

Measurement of current flow produced by an redox rxn

A

Amperometry

27
Q

Measurement of electrical potential due to the activity of free ions

A

Potentiometry

28
Q

Separation is based on relative solubility in an organic solver and an aqueous solvent

A

Partition chromatography

29
Q

Gold standard of analysis

A

Mass spectrophotometey

30
Q

2 types of planar chromatography

A

Paper chromatography

Thin layer chromatography

30
Q

Measures the amt of light emitted by a molecule After excitation by electromagnetic force in a zero background

A

Fluometry

31
Q

An independent analytical method

A

Mass spectrophotometry

32
Q

Quenching lab test

A

Fluometry

33
Q

PCO2 electrode

A

Severinghaus electrode

34
Q

Ph electrode

A

Glass electrode

35
Q

Light source of fluometry

A

Mercury arc and xenon lamp

36
Q

pO2 electrode

A

Clark electrode

37
Q

Electrochemical transducer capable of recepting to one ion

A

Ion selective electrode

39
Q

Electrochemical titration

A

Coulometry

40
Q

Measurement of reduction of lihht due to particle formation

A

Turbidimetry

41
Q

Usually used fir chlorine test in serum, csf, plasma and sweat

A

Coulometry

42
Q

Electrochemistry technique that detects very low analyte lvl

A

Voltametry

44
Q

Fragmentation and ionization of molecules using suitable source of energy

A

Mass spectrophotometry

Clinical Chemistry (6 decks)

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