Other Analytical Methods Flashcards
Excitation of electrons from lower to higher energy
Flame emission photometry
Determines the amt of light blocked
Turbidetry
Light source of of atomic absorption spectrophotometry
Hollow cathode lamp
Determines the amount of scattered light at various angles
Nephelometry
Unknown sample is made to react with a known solution in the presence of an indicator
Volumetric (titrimetric)
Measures light produced by atoms burned by a flame
Flame emission photometry
Standard pH
8.6
Chlorine test
Schales and schales method
Migration of charged particles in an electric field
Electrophoresis
Most common pH buffer
Pheno barbital/ veronal/barbital
Calcium test
Edta titration method
Used to scan and quantitate electrophoretic patterns
Densitometry
PH of tris boric edta
8.7
Stain for proteins and enZyme
Amino black, ponceau s
Ph of veronal
8.6
Stains for fat and lipoproteins
Oil red o / sudan black/ fat red 7B
Stain for csf proteins
Coomassie blue
Measures the absorbance of the molecules
Densitometry
Supporting media for isoelectric focusing
Agarose gel
Polyacrylamide gel
Cellulose acetate
Separates molecules through a pH gradient
Isoelectric focusing
2 forms of chromatography
Planar
Column
2 types of column chromatography
Gas
Liquid chromatography
Separation based on the sign and ionic charge density
Ion exchange chromatography
Chromatography for volatile substances
Gas chromatography
Chromatography that uses lower temp and easier to recover samples
Liquid chromatography
Separates molecules based on the differences in their size and shape
Gel/ gel permeation / gel filtration chromatography
Measurement of current flow produced by an redox rxn
Amperometry
Measurement of electrical potential due to the activity of free ions
Potentiometry
Separation is based on relative solubility in an organic solver and an aqueous solvent
Partition chromatography
Gold standard of analysis
Mass spectrophotometey
2 types of planar chromatography
Paper chromatography
Thin layer chromatography
Measures the amt of light emitted by a molecule After excitation by electromagnetic force in a zero background
Fluometry
An independent analytical method
Mass spectrophotometry
Quenching lab test
Fluometry
PCO2 electrode
Severinghaus electrode
Ph electrode
Glass electrode
Light source of fluometry
Mercury arc and xenon lamp
pO2 electrode
Clark electrode
Electrochemical transducer capable of recepting to one ion
Ion selective electrode
Electrochemical titration
Coulometry
Measurement of reduction of lihht due to particle formation
Turbidimetry
Usually used fir chlorine test in serum, csf, plasma and sweat
Coulometry
Electrochemistry technique that detects very low analyte lvl
Voltametry
Fragmentation and ionization of molecules using suitable source of energy
Mass spectrophotometry