Spectrophotometry

Question 1

What is spectrophotometry?

Answer 1

• Spectrophotometry is a method to measure how much a chemical substance absorbs or transmits light

Question 2

What are the different types of spectrophotometers?

Answer 2

• UV-spectrophotometer uses light over the UV and visible range of the electromagnetic spectrum
• IR spectrophotometer uses light over the IR range of the electromagnetic spectrum

Question 3

What can spectrophotometry be used for?

Answer 3

• Can be used to examine blood and spinal fluid for clinical diagnosis

Question 4

What do the results mean?

Answer 4

• The higher the amount of absorbance means less light is being transmitted