Spectrophotometric Det. of Iron

Question 1

How can we tell hoe much iron there is?

Answer 1

Iron forms several colored complex ions. If you have a ligand that works specifically with iron, then the color will change a slight amount and it can be determined by a spectrophotometer

Question 2

TO get a naked eye visual you must utilize the reaction…

Answer 2

Fe(H2O)6 + 3 phen —> Fe(phen)3 + 6H2O

almost colorless + colorless —> intense orange-red

Question 3

In order to determine the total amount of iron present:

Answer 3

Fe2+ and Fe#+ must both be reduced

Question 4

How do you reduce the Fe?

Answer 4

The addition of hydroxylamine in the form of soluble hydrochloride

Question 5

What acts as a buffer in the system?

Answer 5

ammonium acetate

Question 6

What is the absorbance equation?

Answer 6

A=log(100/%T)

Question 7

How do we know the absorbance is proportional to concentration?

Answer 7

The cuvettes ensure this

Question 8

50 mg of Fe per liter = X ppm

Answer 8

50 ppm

Question 9

What do we leave out of the blank solution?

Answer 9

Fe