Spectophotmetry

Question 1

Electromagnetic spectrum of visible light?

Answer 1

400-700nm

Question 2

Spectroscopy?

Answer 2

Study of radiation emmited or absorbed by a substance

Question 3

Spectrophotometry:

Answer 3

Spectroscopy that soecializies jn visible light (,uv & infra-red)

Question 4

Light source for visible light?

Answer 4

Tungsten halogen

Question 5

Light source for UV light?

Answer 5

Deuterium lamp

Question 6

Cuvette type used for UV light?

Answer 6

Quartz

Question 7

Cuvette type used for visible light?

Answer 7

Plastic or glass

Question 8

Usual width of cuvette (path length)

Answer 8

1 cm

Question 9

Transmittance =

Answer 9

I/I = I/I0

Question 10

Absorbance (A) =

Answer 10

Log (I0/I)

Question 11

What is the range of value for absorbance

Answer 11

0-2

Question 12

What is the Beer-Lambert law

Answer 12

A = ε b c

Question 13

ε ; what does it represent & what’s the unit?

Answer 13

Molar extraction coefficient
L/Mol•Cm

Question 14

What does b represent ?

Answer 14

Path length

Question 15

What does c represent & what’s the unit?

Answer 15

Concentration
Mol/L

Question 16

The relation shape between absorbance and wavelength is ?

Answer 16

Positive correlation until λmax

Question 17

What is the λmax of Quinoneimine?

Answer 17

500nm

Question 18

What’s the absorbance of: 300mg/dl

Answer 18

0.769 (~0.8)

Question 19

What’s the absorbance of: 150mg/dl

Answer 19

~0.4

Question 20

What’s the absorbance of: 75mg/dl

Answer 20

~0.2

Question 21

What’s the absorbance of: Sample A

Answer 21

0.336 (75~150mg/dl)

Question 22

What’s the absorbance of: Sample B

Answer 22

0.179 (<75mg/dl)