Chromatography Flashcards
T or F; Chromatography can be used on a colorless mixture?
True
Why is cellulose used in paper chromatography?
polymer chains with hydroxyl groups, interact strongly with water, absorb well
What are the components of our solvent?
Water , ammonia & methanol
Why do we need to cover the paper inside the beaker during chromatography?
Because the solvent contains ammonia which is volatile
What is nihydrin acetone composed of?
0.2 ninhydrin disolved in acetone
How far did the solvent move in our experiment?
5.7cm
Distance moved by: Proline ?
5.5cm
Distance moved by: Leucine ?
5cm
Distance moved by: Alanine ?
5.2cm
Distance moved by: Aspartic acid ?
4.7cm
Distance moved by: Arginine ?
2.8cm
Distance moved by: Unknown amino acid ?
5.2cm