Specimen transport, acessioning and dissection Flashcards
what are most speciments collected, transported, and stored in
neutral buffered formalin
3 reasons why formalin is best
- cheap and stable
- procedures are validated using it
- tolerant = not damage tissues prolonged
use of gluteratldehyde instead of formalin
PNS and EM specimens
use of bouins solution instead of formalin
trichrome staining or fixing while decalcifying
use of ethanol instead of formalin
when uric acid is resquested
4 ways of using unfixed tissue
fresh frozen
isotonic saline
mechels medium
RPMI
unfixed tissue in isotonic saline purpose
kidney and muscle transport <1 hr
unfixed tissue in michels medium purpose
preserves tissue antigenicity for days = immunofluorescent methods for living conditions
what fixative creates autofluorescence
formaldehyde (formalin), specifically aldehydes
unfixed tissue in RPMI purpose
lyphocyte preserved for lymphoma protocols
what accessioning is needed on paraffin blocks
the specimen number (one Identifier)
what is needed for major descrepancies
physicain takes full responsibility using a waiver document
Decipher what S23-6439-B components
S = surgical sample
23 = year
6439 = tally of specimen recieved in a year
B = more than one container
what are the steps to grossing
set up station
check requisition and sample
dictate
orient tissue - DO BEFORE CUTTING
describe and dissect
what is a representative section
small portion of tissue to represent similar areas
what is a standard section
sections that are always collected
what standard section submitted for appendix
proximal and distal ends
what standard section submitted for placenta
membrane, umbilical cord, and 2 thick sections
what standard section submitted for gallbladder
cystic duct, lymph nodes, and fundus
T/F malignant specimens are grossed by techs
F - PAs do it
what is the max height for cassette
4mm
what is the most common cause of poor tissue processing during grossing
overfilling cassette
what fixative is used for skin biopsies
10% NBF
what disease forms fluid in the vessicles between the dermis and epidermis
bullous pemphigoid
Purpose and tissue layers from shave biopsy
mainly epidermis
papilloma, skin tags, superficial dermatitis
purpose and tissue layers from punch biopsy
epidermis and dermis
iflammatory conditions (-itis)
purpose and tissue layers from elliptical excision
epidermis, dermis, and subcutaneous
melanoma
how long does a skin biopsy need to be to NOT be cut
<0.3cm
