Specimen transport, acessioning and dissection

Question 1

what are most speciments collected, transported, and stored in

Answer 1

neutral buffered formalin

Question 2

3 reasons why formalin is best

Answer 2

1. cheap and stable
2. procedures are validated using it
3. tolerant = not damage tissues prolonged

Question 3

use of gluteratldehyde instead of formalin

Answer 3

PNS and EM specimens

Question 4

use of bouins solution instead of formalin

Answer 4

trichrome staining or fixing while decalcifying

Question 5

use of ethanol instead of formalin

Answer 5

when uric acid is resquested

Question 6

4 ways of using unfixed tissue

Answer 6

fresh frozen
isotonic saline
mechels medium
RPMI

Question 7

unfixed tissue in isotonic saline purpose

Answer 7

kidney and muscle transport <1 hr

Question 8

unfixed tissue in michels medium purpose

Answer 8

preserves tissue antigenicity for days = immunofluorescent methods for living conditions

Question 9

what fixative creates autofluorescence

Answer 9

formaldehyde (formalin), specifically aldehydes

Question 10

unfixed tissue in RPMI purpose

Answer 10

lyphocyte preserved for lymphoma protocols

Question 11

what accessioning is needed on paraffin blocks

Answer 11

the specimen number (one Identifier)

Question 12

what is needed for major descrepancies

Answer 12

physicain takes full responsibility using a waiver document

Question 13

Decipher what S23-6439-B components

Answer 13

S = surgical sample
23 = year
6439 = tally of specimen recieved in a year
B = more than one container

Question 14

what are the steps to grossing

Answer 14

set up station
check requisition and sample
dictate
orient tissue - DO BEFORE CUTTING
describe and dissect

Question 15

what is a representative section

Answer 15

small portion of tissue to represent similar areas

Question 16

what is a standard section

Answer 16

sections that are always collected

Question 17

what standard section submitted for appendix

Answer 17

proximal and distal ends

Question 18

what standard section submitted for placenta

Answer 18

membrane, umbilical cord, and 2 thick sections

Question 19

what standard section submitted for gallbladder

Answer 19

cystic duct, lymph nodes, and fundus

Question 20

T/F malignant specimens are grossed by techs

Answer 20

F - PAs do it

Question 21

what is the max height for cassette

Answer 21

4mm

Question 22

what is the most common cause of poor tissue processing during grossing

Answer 22

overfilling cassette

Question 23

what fixative is used for skin biopsies

Answer 23

10% NBF

Question 24

what disease forms fluid in the vessicles between the dermis and epidermis

Answer 24

bullous pemphigoid

Question 25

Purpose and tissue layers from shave biopsy

Answer 25

mainly epidermis

papilloma, skin tags, superficial dermatitis

Question 26

purpose and tissue layers from punch biopsy

Answer 26

epidermis and dermis

iflammatory conditions (-itis)

Question 27

purpose and tissue layers from elliptical excision

Answer 27

epidermis, dermis, and subcutaneous

melanoma

Question 28

how long does a skin biopsy need to be to NOT be cut

Answer 28

<0.3cm