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Molbio > Specimen Storage Requirements for Nucleic Acid Extraction > Flashcards

Specimen Storage Requirements for Nucleic Acid Extraction Flashcards

1
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?:24 hours

A

22°–25°C

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2
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?:72 hours *

A

2°–8°C

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3
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?: At least 1 year *

A

–20°C

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4
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?:More than 1 year *

A

–70°C

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5
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
22°–25°C : TIME?

A

24 hours

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6
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
2°–8°C : TIME?

A

72 hours *

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7
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
–20°C : TIME?

A

At least 1 year *

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8
Q

DNA

Whole blood, buffy coat,
bone marrow, fluids
–70°C : TIME?

A

More than 1 year *

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9
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
22°–25°C

A

Not recommended

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10
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
2°–8°C: TIME

A

2–4 hours *

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11
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
–20°C: TIME

A

2–4 weeks *

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12
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
–70°C : TIME

A

More than 1 year *

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13
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP ; 2–4 hours *

A

2°–8°C

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14
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP ; –20°C

A

2–4 weeks *

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15
Q

RNA

Whole blood, buffy coat,
bone marrow, fluids
TIME ; –70°C

A

More than 1 year *

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16
Q

RNA

Fluids collected in specialty
22°–25°C ; TIME

A

5 days

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17
Q

RNA

Fluids collected in specialty
TEMP ; 5 days

A

22°–25°C

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18
Q

RNA

Fluids collected in specialty
TEMP ;TIME

A

22°–25°C : 5 days

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19
Q

RNA

RNA protection tubes
2°–8°C ; TIME

A

7 days

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20
Q

RNA

RNA protection tubes
–20°C ; TIME

A

2–4 weeks

21
Q

RNA

RNA protection tubes
–70°C ; TIME

A

At least 7 months

22
Q

RNA

RNA protection tubes
TEMP ; 7 days

A

2°–8°C

23
Q

RNA

RNA protection tubes
TEMP ; 2–4 weeks

A

–20°C

24
Q

RNA

RNA protection tubes
TEMP ; At least 7 months

A

–70°C

25
Q

RNA

Tissue
22°–25°C

A

Not recommended

26
Q

RNA

Tissue
2°–8°C

A

Not recommended

27
Q

RNA

Tissue
–20°C

A

Not recommended

28
Q

RNA

Tissue
–70°C ; TIME

A

At least 2 years

29
Q

RNA

Tissue
–140°C (nitrogen vapor) ; TIME

A

At least 2 years

30
Q

RNA

Tissue
TEMP ; At least 2 years

A

–70°C

31
Q

RNA

Tissue
TEMP ; At least 2 years

A

–140°C (nitrogen vapor)

32
Q

RNA

Cell lysates in GITC
TEMP ; 1–2 weeks

A

22°–25°C

33
Q

RNA

Cell lysates in GITC
TEMP ; TIME

A

22°–25°C ; 1–2 weeks

34
Q

RNA

Cell lysates in GITC
22°–25°C ; TIME

A

1–2 weeks

35
Q

RNA

Cell lysates in RNA storage solution (Ambion)
TEMP ; 1 week

A

22°–25°C

36
Q

RNA

Cell lysates in RNA storage solution (Ambion)
22°–25°C ; TIME

A

1 week

37
Q

RNA

Cell lysates in RNA storage solution (Ambion)
TEMP ; 1 month

A

2°–8°C

38
Q

RNA

Cell lysates in RNA storage solution (Ambion)
TEMP ; More than 1 year

A

–20°C

39
Q

RNA

Cell lysates in RNA storage solution (Ambion)
2°–8°C ; TIME

A

1 month

40
Q

RNA

Cell lysates in RNA storage solution (Ambion)
–20°C ; TIME

A

More than 1 year

41
Q

RNA

Microorganisms in culture
22°–25°C ; TIME

A

24 hours

42
Q

RNA

Microorganisms in culture
2°–8°C ; TIME

A

72 hours

43
Q

RNA

Microorganisms in culture
–20°C ; TIME

A

2–4 weeks

44
Q

RNA

Microorganisms in culture
–70°C ; TIME

A

More than 1 year

45
Q

RNA

Microorganisms in culture
TEMP ; 24 hours

A

22°–25°C

46
Q

RNA

Microorganisms in culture
TEMP ; 72 hours

A

2°–8°C

47
Q

RNA

Microorganisms in culture
TEMP ; 2–4 weeks

A

–20°C

48
Q

RNA

Microorganisms in culture
TEMP ; More than 1 year

A

–70°C

Molbio (5 decks)

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