Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Molbio > CHAPTER 1 > Flashcards

CHAPTER 1 Flashcards

Liquid handling tool selection

1
Q

Meant for general use with aqueous solutions

A

Air displacement pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Two types of pipettes

A

Air displacement pipettes
Positive displacement pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Used for highly viscous and volatile
liquids

A

Positive displacement pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Highly accurate for standard pipetting applications

A

Air displacement pipetting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

May affect the performance of air displacement pipettes

A

Temperature
Atmospheric pressure
Specific gravity
Viscosity of the solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

This is based on direct contact of the piston with the liquid

A

Positive displacement pipetting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In air displacement pipettes the tips contain both the cylinder/capillary and the piston (T/F)

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

In positive displacement pipettes, a certain volume of air remains between the piston and the liquid. (T/F)

A

F

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

In positive displacement pipetting, the piston is in direct contact with the liquid. (T/F)

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The type of experiment you are performing and the physical properties of the liquid will determine the correct pipette tip to use. (T/F)

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

A multi-purpose tip for many laboratory applications with a variety of performance requirements that range from high accuracy to reagent dispensing with greater tolerance

A

Standard Tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

This is available for applications demanding the highest level of purity

A

Sterile standard tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Beneficial when the assay is sensitive to cross-contamination, or if the sample can contaminate the lower part of the pipette

A

Filter tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

This prevents liquid from accidentally splashing the inside of the pipette, and reduces aerosols from penetrating the pipette tip cone during pipetting

A

Filter tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

This is recommended for low volume applications in genetic studies, forensics, PCR, and radioisotope sampling

A

Filter tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Filter tips are recommended for low volume applications in …

A

Genetic studies
Forensics
PCR
Radioisotope sampling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Filter tips are available with either … – both of which are designed to prevent cross-contamination

A

Self-sealing barrier
Standard filter tips

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Filter tips are available with either self-sealing barrier or standard filter tips – both of which are designed to

A

Prevent cross-contamination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

This allow you to access the bottom of test tubes, reagent bottles, flasks, and other vessels without touching the shaft of the pipette against the side of the tube.

A

Extended length tips

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

This adds a layer of security to protect samples, and virtually eliminates the chance of carryover contamination.

A

Extended length tips

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

The longer tip length allows you to reach the bottom of long or narrow vessels that standard tips cannot reach (T/F)

A

T

22
Q

The shorter tip length allows you to reach the bottom of long or narrow vessels that standard tips cannot reach (T/F)

A

F

23
Q

This is designed for unique pipetting applications to save time, reduce contamination, and increase accuracy, precision, and productivity

A

Specialty tips

24
Q

Specialty tips are designed for unique pipetting applications to

A

Save time
Reduce contamination
Increase accuracy, precision, and productivity

25
Q

Utilizing polymer technology makes the inner surface of the pipette tip more hydrophobic, resulting in a significant reduction in sample loss due to adhesion.

A

Low retention pipette tips

26
Q

Benefits include improved sample delivery and conservation of expensive reagents

A

Low retention pipette tips

27
Q

Benefits of low retention pipette tips

A

Improved sample delivery
Conservation of expensive reagents

28
Q

This tip feature a distal end orifice that is nearly 70 percent larger than that of a standard pipette tip

A

Wide orifice tip

29
Q

This provide the added flexibility required for handling difficult-to-pipette samples

A

Wide orifice tip

30
Q

This is designed for researchers working with macromolecules like genomic DNA and are especially critical for transferring fragile cellular samples such as macrophages, hybridomas, and hepatocytes, as well as other viscous materials

A

Wide orifice tip

31
Q

Tips

A

Standard tips
Filter tips
Extended length pipete tips
Long retention pipette tips
Specialty tips
Wide orifice tips
Gel Loading Tips
Solvent Safe Carbon Filtered Tips
Individually Wrapped Tips

32
Q

Tips used for agarose gels

A

Round gel loading tips

33
Q

Tips used for your polyacrylamide gels

A

Specialized Ultra Round and Ultra Flat
gel tips

34
Q

Best solution for handling the pipetting rigors of Combinatorial Chemistry.

A

Solvent Safe Carbon Filtered Tips

35
Q

These specialized tips keep strong acids, bases, and aggressive organic solvents from causing pipette failures and critical inaccuracies.

A

Solvent Safe Carbon Filtered Tips

36
Q

This is for extremely sensitive applications

A

Individually Wrapped Tips

37
Q

This is deal for extremely sensitive applications requiring strict aseptic conditions

A

Individually Wrapped Tips

38
Q

Due to their versatility and wide volume range these are the most used laboratory instruments

A

Variable volume air displacement single-channel pipettes

39
Q

Most commonly used in microplate applications, such as ELISA, PCR, or cell culture.

A

Multichannel pipettes

40
Q

Offer instant usability for small scale multichannel work.

A

Manual multichannel pipettes

41
Q

These are available as 8- or 12-channel versions to work with 96-well microplates, and as a 16-channel version to work with 384-well microplates.

A

Multichannel pipettes

42
Q

These are versatile laboratory workhorses that can be programmed to perform most laboratory tasks.

A

Electronic pipettes

43
Q

The most commonly used function of electronic pipettes is the ..

A

Aliquoting of a reagent into multiple doses - MULTIDISPENSING

44
Q

By using a multichannel electronic pipette and repeat dispensing modes in microplate filling, the time needed for filling the plate can be reduced from several minutes to less than a minute. (T/F)

A

T

45
Q

Electronic pipettes that offer adjustable tip spacing increase efficiency in samples transfers to move several samples at once between different labware formats. (T/F)

A

T

46
Q

These are used in cell and tissue culture applications, and
in general laboratory liquid dosage when more than 1 ml volumes are pipetted.

A

Serological pipets

47
Q

These are made of glass or polystyrene. Plastic, disposable pipets are useful in applications where sterility is a requirement.

A

Serological pipets

48
Q

Help to aspirate and dispense liquids accurately
and with precision.

A

Serological Pipet Fillers

49
Q

In serologic pipets, the speed of both aspiration and dispensing can be adjusted separately to work with a variety of liquids. (T/F)

A

T

50
Q

This is ideal for simple repetitive tasks with a dispensing range up to 5 ml.

A

Manual Stepper pipettes

51
Q

This is a reliable and easy tool for dispensing reagents directly from the reagent bottle.

A

Bulk reagent dispenser

52
Q

This offers speed and accuracy with no extra work steps in everyday liquid dispensing.

A

Bulk reagent dispenser

Molbio (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions