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PARASITOLOGY > SPECIMEN & SPECIMEN PROCESSING > Flashcards

SPECIMEN & SPECIMEN PROCESSING Flashcards

1
Q

most common submitted sample

A

stool

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2
Q

what are the toxic substances to intestinal protozoans

A

-mineral oil
-antibiotics
-bismuth
-antimalarial agents
-Non-absorbable antimalarial antidiarrheal preparation

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3
Q

formed stool should be examined within how many hours/minutes

A

24hr

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4
Q

watery stool should be examined within how many hours or minutes

A

30 minutes

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5
Q

semi-formed stool should be examined within how many hours

A

1 hour

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6
Q

recommended fixative to specimen volume ratio

A

3:1

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7
Q

stool to preservative ratio

A

1:3

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8
Q

used to preserved fresh stool/fresh fecal specimen in preparation for staining stool smears
-“gold standard”

A

Schaudinn’s solution

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9
Q

all-purposed fixative; usually buffered with sodium phosphate to preserve morphological characteristic

A

Formalin

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10
Q

% formalin solution recommended for protozoan cysts

A

5% concentration

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11
Q

% formalin solution recommended for helminthic eggs

A

10% concentration

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12
Q

-useful for fixation of intestinal protozoans, helminth eggs and larvae
-components both fix and provide stain color

A

merthiolate iodine-formalin (MIF)

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13
Q

-preservation of protozoan cyst and trophozoites for permanent staining
-plastic resin that serves to adhere a stool sample onto a slide

A

polyvinyl alcohol (PVA)

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14
Q

stool preservatives that contains mercury :

A

-schaudinn’s solution
-merthiolate iodine formalin (MIF)
-polynvinyl alcohol (PVA)

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15
Q

used to recover enterobious vermicularis, Taenia spp, and schistosoma mansoni egg

A

Perianal swab

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16
Q

a piece of transparent adhesive tapr is pressed firmly against perianal skin, and the adhesive surface of the tape is spread on a glass slide

A

cellulose tape or scotch tape method

17
Q

how many negative tapes are required to rule out the infection

A

4-6 consecutive negative

18
Q

next to feces, it is where the largest number of parasites found

A

blood

19
Q

the stain of choice

A

Giemsa stain

20
Q

stain if often used to stain the microfilarial sheath

A

delafield’s heamtoxylin

21
Q

a request for the examination of this specimen is always STAT request

A

blood

22
Q

it allows the malarial parasite to be detected even in low parasitemia
-about 30 times more sensitive can detect about 20 parasite/uL of blood

A

stained smear: thick films

23
Q

it serves as the quality control on the stained blood film

A

WBC

24
Q

it is routinely used for parasite identification to the species level

A

STAINED SMEAR: THIN FILM

PARASITOLOGY (2 decks)

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