Specimen Collection

Question 0

More cysts are found in what type of stool?

Answer 0

Formed

Question 1

How are stool specimens collected?

Answer 1

Fresh stool in wide mouth container & examined within 30-60min

Question 2

More trophozoites are found in what type of stool?

Answer 2

Liquid

Question 3

3 types of stools:

Answer 3

Liquid soft or formed

Question 4

Permanently stained smear DNA-PCR

Answer 4

Polyvinyl alcohol (PVA)

Question 5

Formalin-ethyl acetate concentration, direct wet mount, & immunoassays

Answer 5

10% formalin

Question 6

Permanently stained smears and concentration

Answer 6

Sodium acetate-acetic acid- formalin (SAF)

Question 7

Concentration and direct wet mount

Answer 7

Mentholated-iodine-formalin (MIF)

Question 8

Concentration, direct wet mount, permanently stained smears, & immunoassays

Answer 8

Single-vial systems

Question 9

3 times of preparation for liquid and aid stool:

Answer 9

Direct wet mounts, concentrates, & permanent stained smears

Question 10

Microscopic examination in saline?

Answer 10

Motility of trophozoites & appear more refractive

Question 11

Microscopic examination in iodine?

Answer 11

Highlights internal structures of parasite & destroys trophozoite motility & stains glycogen

Question 12

What is the gold standard for parasite ID?

Answer 12

Stained smears

Question 13

Eggs, cysts, & trophozoites can be:

Answer 13

Low in #, confused with fecal debris, & concentrated for improved detection

Question 14

Sedimentation

Answer 14

Solutions with specific gravity lower than parasitic organisms, use centrifugation to pellet sediment, organisms are concentrated in sediment, widely used

Question 15

Iron hematoxylin (permanently stained smear)

Answer 15

High definition of parasitic structures, difficult to do & variability

Question 16

Trichromatic stain (permanently stained smear)

Answer 16

Cytoplasm of cyst & trophozoites are blue-green tinged with purple, nuclear chromatin, ingested RBCs appear red or red-purple, background is green & widely used

Question 17

Flotation

Answer 17

Solutions with higher specific gravity than parasitic organisms, organisms are concentration in the flotation, eliminates some fecal debris, may miss some sense organisms of settling occurs

Question 18

How many fields do u scan for parasites?

Answer 18

10-15

Question 19

Modified acid-fast stain

Answer 19

Detects oocysts, stains magenta against a blue background

Question 20

Oocyst

Answer 20

Encysted zygotic can stage in the life cycle of some sporozoans

Question 21

What is the cellophane tape test used for?

Answer 21

Examining Enterobius vermicularis is (pinworm)

Question 22

What is the string test used for?

Answer 22

Testing for Giardia lam loa or Strongyloides stereo rails in the duodenum & jejunum

Question 23

For Malaria and Babesia collect samples at?

Answer 23

6 hr intervals over 36-48 hrs