Specifically describe how the lac operon (enzyme induction) and trp operon (enzyme repression) control gene expression. Use a diagram. Flashcards
1
Q
Lac Operon: Enzyme Induction
A
The lac operon helps break down lactose.
- When lactose is absent, the lac repressor binds to the operator, stopping RNA polymerase from transcribing the genes.
- When lactose is present, it acts as an inducer, binding to the repressor and inactivating it.
- This lets RNA polymerase bind to the promoter and start transcribing the genes to break down lactose.
This is enzyme induction, where lactose triggers the production of enzymes.
2
Q
Comparison
A
- Lac Operon: Off until lactose turns it on. Lactose helps make enzymes to break it down.
- Trp Operon: On until tryptophan turns it off. Tryptophan stops its own production when there’s enough.
Lac = Stopped until activated
Trp = Active until stopped
3
Q
Trp Operon: Enzyme Repression
A
The trp operon controls the production of tryptophan.
- When tryptophan is absent, the trp repressor is inactive, and RNA polymerase can transcribe the genes to make tryptophan.
- When tryptophan is present, it acts as a corepressor, activating the repressor.
- The active repressor binds to the operator, blocking RNA polymerase from transcribing the genes.
This is enzyme repression, where tryptophan stops the production of more tryptophan when there’s enough.