SPECIAL STAINS Flashcards
Sudan black*
Lipids : blue-black
Sudan IV (SCHARLACH R)*
Lipids (TAG) = red
* MOST COMMONLY USED
Oil red o*
Fat: BRILLIANT RED
Osmic acid stain*
Fats : black
Nile blue sulfate mtd
Neutral fats: pinkish red/Cholestrin esters and cholestrin
Fatty acids: lt red
Cerebrosides: lt blue
Fatty acids and soap: Deep blue to violet
Toluidine blue - acetone mtd
Sulfatide: metachromatic red-brown or yellow
Birohydride -PAS MTD
Gangliosides - red
CHO STAIN
forms H bonds
Selective and specific for glycogen
BEST CARMINE
Cho stain: Stain of choice for glycogen
PAS with diastase ctrl
Protein stain:
Histones and protaminase - green
Alkaline fast- green mtd
Special stain for Nucleic acid
MOST RELIABLE and SPECIFIC STAIN FOR DNA
Fuelgen technique
Special stain for Nucleic acid
RNA (nucleoli) = red
DNA (chromatin) = green
Methyl green - pyronin
Special stain for Nucleic acid
DNA: yellow-green fluorescence
RNA: brick to orange red fluorescence
Acridine orange fluorescent stain
Tse pigment & Deposits stain
Melanin and argentaffin cell granules - black
Masson-Fontana technique
Tse pigment & Deposits stain
Calcium- black
Von kossa’s silver nitrate mtd
