1

Question 1

Type of cell death involving CASPASE enzyme

Answer 1

APOPTOSIS

Question 2

Surgical connection between two structure

Answer 2

Anastomosis

Question 3

Organs removed and dissected individually un the body (MOST WIDELY USED)

Answer 3

Technology of RUDOLF VIRCHOW

Question 4

IN-SITU DISSECTION in part combined with EN BLOC TECHNIQUE

Answer 4

Technology of CARL VON ROKITANSKY

Question 5

EN BLOC technique

Answer 5

Technology of ANTON GHON

Question 6

EN MASSE technique

Answer 6

Technology of MAURICE LETULLE

Question 7

Cold knife procedure:

Knife:
Tse:
Environment:

Answer 7

Knife: -40 to -60’C
Tse: -5 to -10’C
Environment: 0 to -10’C

Question 8

Cryostat temp.

Answer 8

-18 to -20’C

Question 9

Freeze drying temp

Answer 9

Quenching: -160’C

Sublimation: -40’C

Question 10

Fixation temp

Answer 10

Sx’cal spx: RT

Histochem, EM: 0-4’C

Question 11

Formol Calcium temp

Answer 11

4’C

Question 12

Vacuum embedding temp

Answer 12

2-4’C above the melting pt of wax

Question 13

Automated impregnation temp

Answer 13

3’C above the melting pt of wax

Question 14

*MANUAL IMPREGNATION temp

Answer 14

2-5’C above the melting pt of wax (55’C to 60’C)

Question 15

EMBEDDING

Answer 15

5-10’C above the melting pt of wax

Question 16

Floatation water bath*

Answer 16

6-10’C BELOW THE MELTING OF WAX (45’C to 50’C)

Question 17

Melting pt of wax:

Lab temp: 20-24’C

Answer 17

54-58’C (56’C)

Question 18

Melting pt of wax:

Lab temp: 15-18’C

Answer 18

50-54’C

Question 19

Water present in paraffin wax can be removed by:

Answer 19

Heating wax at 100-105’C

Question 20

Gives the fastest result for paraffin wax impregnation

Answer 20

Vacuum embedding mtd

Question 21

In double embedding, tse is first infiltrated w/ ____, then embedded w/ ____

Answer 21

Celloidin

Paraffin

Question 22

Are used for INFILTRATION and EMBEDDING

Answer 22

CGP3

Celloidin
Gelatin

Paraffin
Paraffin subs
Plastic

Question 23

Autotechnicon can perform: (4)

Answer 23

Fixation
Dehydration
Clearing
Infiltration

DIC -F

Question 24

Sliding microtome

Answer 24

1789: Adams

Question 25

Rocking (Cambridge) microtome

Answer 25

Paldwell Trefall (1881)

Question 26

Rotary microtome

Answer 26

Minot (1885-1886)

Question 27

Freezing microtome

Answer 27

Queckett (1848)

Question 28

Simplest microtome

Answer 28

Rocking (Cambridge)

Question 29

Microtome incorporated in the Cryostat machine

Answer 29

Rotary (Minot)

Question 30

Bevel angle

Answer 30

27’-32’

Question 31

Clearance angle (knife to tse block)

Answer 31

0-15’ (5-10’)

Question 32

Knife recommended for cutting paraffin-embedded sections on rotary microtome

Answer 32

Biconcave knife

Question 33

Removal of nicks ( heel to toe, EDGE 1st)

Answer 33

Honing

Question 34

Honing stones (3)

Answer 34

Belgium yellow Arkansas Fine carborundum FAB

Question 35

Removal of burrs (toe to heel, EDGE LAST)

Answer 35

Stropping

Question 36

Used in stropping

Answer 36

Paddle strop (made of horse leather)

Question 37

Causes of tse brittleness

Answer 37

Prolonged fixation Dehydration Clearing Paraffin infiltration

Question 38

Will make the tse opaque and difficult to cut due to presence of alcohol

Answer 38

INCOMPLETE CLEARING

Question 39

Examples of mordants

Answer 39

K alum in Ehlich’s hematoxylin Iron in Weigert’s hematoxylin

Question 40

Sealing the margins of the coverslip

Answer 40

Ringing

Question 41

Source of MONOCLONAL AB

Answer 41

Mice

Question 42

SOURCE of POLYCLONAL AB for immunohistochem techniques

Answer 42

Rabbit 🐰 * ``` ✔️ GOAT 🐐 ✔️ PIG 🐖 ✔️SHEEP 🐑 ✔️ HORSE 🐎 ✔️ GUINEA PIG 🐹 ```

Question 43

Color of AMINOETHYLCARBAZOLE (AEC)

Answer 43

Red

Question 44

Color of DIAMINOBENZIDINE (DAB)

Answer 44

BROWN

Question 45

Consists of cellular material which gas been transferred from 1 smear to another, usually DURING STAINING

Answer 45

Floater

Question 46

Floaters may lead to _____

Answer 46

False ➕ if POSITIVE FLOATERS adhere to negative smear

Question 47

Best type of fixative for EXFOLIATIVE CYTOLOGY

Answer 47

Equal parts of ETHANOL:ETHER *highly flammable

Question 48

Most commonly used fixative for EXFOLIATIVE CYTOLOGY

Answer 48

95% ETHYL ALCOHOL

Question 49

Mtd of choice for EXFOLIATIVE CYTOLOGY

Answer 49

Papanicolau smear * difference from Mod. PAP mtd: mod has no BISMARCK BROWN

Question 50

Pale, pink cytoplasm with dark PYKNOTIC NUCLEUS | Under estrogen influence, TRUE ACIDOPHILIA is observed

Answer 50

Mature superficial cell

Question 51

Medium sized POLYHEDRAL/ELONGATED cells with VACUOLATED CYTO

Answer 51

Intermediate cells

Question 52

BOAT-SHAPED 🚣‍♀️ | strong tendency to FOLD or CURL on edges

Answer 52

NAVICULAR (INTERMEDIATE)

Question 53

Contains GLYCOGEN that pushes NUCLEUS ASIDE

Answer 53

PREGNANCY CELL (intermediate)

Question 54

Resembles FRIED-EGGS with SUNNY SIDE UP | ⬆️ in MENOPAUSAL WOMEN

Answer 54

PARABASAL CELLS

Question 55

Groups of 3 or more cells found during menstruation

Answer 55

ENDOMETRIAL CELLS

Question 56

May present a HONEYCOMB 🍯 APPEARANCE when viewed on end

Answer 56

ENDOCERVICAL GLANDULAR CELL

Question 57

For samples of ENDOCERVICAL CANAL

Answer 57

ENDOCERVICAL BRUSH

Question 58

For HYSTERECTOMY PXS

Answer 58

VAGINAL SCRAPE

Question 59

*For Hormonal EVALUATION

Answer 59

*Lateral vaginal scrape

Question 60

For localization of VAGINAL ADENOSIS

Answer 60

Four quadrant vaginal scrape

Question 61

For detection of HERPETIC LESIONS OF CARCINOMA

Answer 61

VULVAR SCRAPE

Question 62

Equipments for VAGINAL ASPIRATION

Answer 62

Glass pipette | Rubber bulb

Question 63

Equipments for SWAB SMEAR

Answer 63

Ayre’s spatula

Question 64

Equipments for ENDOCERVICAL or ENDOMETRIAL ASPIRATIONS

Answer 64

Laryngeal cannula attached to a 10-cc syringe

Question 65

Nuclear stain (Counterstains) Red: NSCH BLUE: MTC

Answer 65

``` Red: Neutral red Safranin O Carmine Hematoxylin ``` Blue: Methylene blue Toluidine Blue Celestine blue

Question 66

Cytoplasmic stains (counterstain) Red: PEE Yellow: POR Green: 2LG’s

Answer 66

Red: Phloxine B Eosin Y Eosin B Yellow: Picric acid Orange G Rose Bengal* Green: Lt Green LISSAMINE green