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HISTOPATH > 1 > Flashcards

1 Flashcards

1
Q

Type of cell death involving CASPASE enzyme

A

APOPTOSIS

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2
Q

Surgical connection between two structure

A

Anastomosis

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3
Q

Organs removed and dissected individually un the body (MOST WIDELY USED)

A

Technology of RUDOLF VIRCHOW

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4
Q

IN-SITU DISSECTION in part combined with EN BLOC TECHNIQUE

A

Technology of CARL VON ROKITANSKY

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5
Q

EN BLOC technique

A

Technology of ANTON GHON

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6
Q

EN MASSE technique

A

Technology of MAURICE LETULLE

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7
Q

Cold knife procedure:

Knife:
Tse:
Environment:

A

Knife: -40 to -60’C
Tse: -5 to -10’C
Environment: 0 to -10’C

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8
Q

Cryostat temp.

A

-18 to -20’C

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9
Q

Freeze drying temp

A

Quenching: -160’C

Sublimation: -40’C

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10
Q

Fixation temp

A

Sx’cal spx: RT

Histochem, EM: 0-4’C

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11
Q

Formol Calcium temp

A

4’C

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12
Q

Vacuum embedding temp

A

2-4’C above the melting pt of wax

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13
Q

Automated impregnation temp

A

3’C above the melting pt of wax

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14
Q

*MANUAL IMPREGNATION temp

A

2-5’C above the melting pt of wax (55’C to 60’C)

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15
Q

EMBEDDING

A

5-10’C above the melting pt of wax

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16
Q

Floatation water bath*

A

6-10’C BELOW THE MELTING OF WAX (45’C to 50’C)

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17
Q

Melting pt of wax:

Lab temp: 20-24’C

A

54-58’C (56’C)

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18
Q

Melting pt of wax:

Lab temp: 15-18’C

A

50-54’C

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19
Q

Water present in paraffin wax can be removed by:

A

Heating wax at 100-105’C

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20
Q

Gives the fastest result for paraffin wax impregnation

A

Vacuum embedding mtd

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21
Q

In double embedding, tse is first infiltrated w/ ____, then embedded w/ ____

A

Celloidin

Paraffin

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22
Q

Are used for INFILTRATION and EMBEDDING

A

CGP3

Celloidin
Gelatin

Paraffin
Paraffin subs
Plastic

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23
Q

Autotechnicon can perform: (4)

A

Fixation
Dehydration
Clearing
Infiltration

DIC -F

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24
Q

Sliding microtome

A

1789: Adams

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25
Q

Rocking (Cambridge) microtome

A

Paldwell Trefall (1881)

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26
Q

Rotary microtome

A

Minot (1885-1886)

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27
Q

Freezing microtome

A

Queckett (1848)

28
Q

Simplest microtome

A

Rocking (Cambridge)

29
Q

Microtome incorporated in the Cryostat machine

A

Rotary (Minot)

30
Q

Bevel angle

A

27’-32’

31
Q

Clearance angle (knife to tse block)

A

0-15’ (5-10’)

32
Q

Knife recommended for cutting paraffin-embedded sections on rotary microtome

A

Biconcave knife

33
Q

Removal of nicks ( heel to toe, EDGE 1st)

A

Honing

34
Q

Honing stones (3)

A

Belgium yellow
Arkansas
Fine carborundum

FAB

35
Q

Removal of burrs (toe to heel, EDGE LAST)

A

Stropping

36
Q

Used in stropping

A

Paddle strop (made of horse leather)

37
Q

Causes of tse brittleness

A

Prolonged fixation
Dehydration
Clearing
Paraffin infiltration

38
Q

Will make the tse opaque and difficult to cut due to presence of alcohol

A

INCOMPLETE CLEARING

39
Q

Examples of mordants

A

K alum in Ehlich’s hematoxylin

Iron in Weigert’s hematoxylin

40
Q

Sealing the margins of the coverslip

A

Ringing

41
Q

Source of MONOCLONAL AB

A

Mice

42
Q

SOURCE of POLYCLONAL AB for immunohistochem techniques

A

Rabbit 🐰 *

✔️ GOAT 🐐 
✔️ PIG 🐖 
✔️SHEEP 🐑 
✔️ HORSE 🐎 
✔️ GUINEA PIG 🐹
43
Q

Color of AMINOETHYLCARBAZOLE (AEC)

A

Red

44
Q

Color of DIAMINOBENZIDINE (DAB)

A

BROWN

45
Q

Consists of cellular material which gas been transferred from 1 smear to another, usually DURING STAINING

A

Floater

46
Q

Floaters may lead to _____

A

False ➕ if POSITIVE FLOATERS adhere to negative smear

47
Q

Best type of fixative for EXFOLIATIVE CYTOLOGY

A

Equal parts of ETHANOL:ETHER

*highly flammable

48
Q

Most commonly used fixative for EXFOLIATIVE CYTOLOGY

A

95% ETHYL ALCOHOL

49
Q

Mtd of choice for EXFOLIATIVE CYTOLOGY

A

Papanicolau smear

  • difference from Mod. PAP mtd: mod has no BISMARCK BROWN
50
Q

Pale, pink cytoplasm with dark PYKNOTIC NUCLEUS

Under estrogen influence, TRUE ACIDOPHILIA is observed

A

Mature superficial cell

51
Q

Medium sized POLYHEDRAL/ELONGATED cells with VACUOLATED CYTO

A

Intermediate cells

52
Q

BOAT-SHAPED 🚣‍♀️

strong tendency to FOLD or CURL on edges

A

NAVICULAR (INTERMEDIATE)

53
Q

Contains GLYCOGEN that pushes NUCLEUS ASIDE

A

PREGNANCY CELL (intermediate)

54
Q

Resembles FRIED-EGGS with SUNNY SIDE UP

⬆️ in MENOPAUSAL WOMEN

A

PARABASAL CELLS

55
Q

Groups of 3 or more cells found during menstruation

A

ENDOMETRIAL CELLS

56
Q

May present a HONEYCOMB 🍯 APPEARANCE when viewed on end

A

ENDOCERVICAL GLANDULAR CELL

57
Q

For samples of ENDOCERVICAL CANAL

A

ENDOCERVICAL BRUSH

58
Q

For HYSTERECTOMY PXS

A

VAGINAL SCRAPE

59
Q

*For Hormonal EVALUATION

A

*Lateral vaginal scrape

60
Q

For localization of VAGINAL ADENOSIS

A

Four quadrant vaginal scrape

61
Q

For detection of HERPETIC LESIONS OF CARCINOMA

A

VULVAR SCRAPE

62
Q

Equipments for VAGINAL ASPIRATION

A

Glass pipette

Rubber bulb

63
Q

Equipments for SWAB SMEAR

A

Ayre’s spatula

64
Q

Equipments for ENDOCERVICAL or ENDOMETRIAL ASPIRATIONS

A

Laryngeal cannula attached to a 10-cc syringe

65
Q

Nuclear stain (Counterstains)

Red: NSCH
BLUE: MTC

A 
Red:
Neutral red
Safranin O
Carmine
Hematoxylin

Blue:
Methylene blue
Toluidine Blue
Celestine blue

66
Q

Cytoplasmic stains (counterstain)

Red: PEE
Yellow: POR
Green: 2LG’s

A

Red:
Phloxine B
Eosin Y
Eosin B

Yellow:
Picric acid
Orange G
Rose Bengal*

Green:
Lt Green
LISSAMINE green

HISTOPATH (2 decks)

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