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Histopath Midterms > Special Stains > Flashcards

Special Stains Flashcards

1
Q

It is term “special” because they are dyes used to stain particular tissues, structures or microorganisms that may not be visible by routine H&E staining.

A

Histochemical stains

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2
Q

It can determine the presence or absence (qualitative) and amount (quantitative).

A

Histochemical stains

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3
Q

the color of particular tissue elements using one stain is different from another type of stain.

A

Histochemical stains

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4
Q

Immunohistochemical stains detects cellular constituents specifically proteins (antigen) thru immunologic principle.

A

Histochemical stains

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5
Q

PAS activity in inhibit by

A

Osmic Acid
Chromates
Permanganates

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5
Q

Other sugars that PAS stain are

A

Glucose
Galactose
Mannose
Methylpentose
Fructose
Hexokinase

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5
Q

resulting color of PERIODIC ACID SCHIFF (PAS)

A

Magenta Red or purplish-pink

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5
Q

What are the Special Stains

A

Carbohydrates (glycogen and mucin)
Fats and lipids
Protein, enzymes and nucleic acids
Connective tissue
Muscle and bone
Central nervous system
Pigments and deposits

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6
Q

Used to stain carbohydrates in histochemical staining.

A

PERIODIC ACID SCHIFF (PAS)

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6
Q

PAS with Diastase Method
Color Result for
Nuclei -
Glycogen -
Control -

A

Black
Red
only the nuclei are stained

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7
Q

Suitable temperature for staining should be

A

25C and above

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8
Q

stain Most suitable in celloidin section

A

Best Carmine Method

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8
Q

Stain can be used in paraffin, frozen or cryostat sections.

A

Perodic Acid Schiff (PAS)

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8
Q

Used Diastase (human saliva) or 0.1 % malt diastase serves as a control.

A

PAS with Diastase Method

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9
Q

stain Highly specific for glycogen

A

Best Carmine Method

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10
Q

Best Carmine Method
Color results for
Nuclei -
Glycogen -
Mucin, fibrin -

A

Blue or Grayish Blue
Bright red Granules
Wear red

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11
Q

Most suitable in celloidin section aka

A

Langhan’s Iodine method

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11
Q

Langhan’s Iodine method also known as

A

Carleton’s modification

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12
Q

This stain is obsolete and not specific

A

Langhan’s Iodine method

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13
Q

Color results for Azure A
Acid mucopolysaccharides -
Other tissue components -

A

Crimson or red violet
Shades of blue

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13
Q

Langhan’s Iodine Method color results for
Glycogen -
Tissue Constituents -

A

Mahogany Brown
Yellow

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14
Q

Color results for Hale’s Dialyzed (Colloidal) Iron Technique
Acid Mucin -
Nuclei -

A

Dark blue
Red

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14
Q

Stains for Glycogen

A

Periodic Acid Schiff (PAS)
Best Carmine
Langhan’s Iodine Method

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15
Q

Stains for Mucin

A

Azure A
Methachromatic Toluidine Blue
Alcian Blue Technique
Gomori’s Aldehyde Fucshin Stain
Mucicarmine Stain
Hale’s Dialyzed (colloidal) Iron Technique
Fluorescent Acridine Orange Technique (immunofluoresnce)

MAG MAH F

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16
Metachromatic stain for acid mucoploysaccharides.
Azure A
17
Color results for Alcian Blue Technique Acid mucin- Nuclei -
Blue Red
18
Color results for Metachromatic Toluidine Blue Glycosaminoglycans - Tissue background-
Red Purple Blue
19
Color results for Gomori’s Aldehyde Fucshin Stain Sulfated mucins- Carboxylated Mucin -
Purple Blue
20
Color results for Mucicarmine Stain Mucins - Nuclei - Background -
Red Blue Unstained
21
A fluorochrome used to demonstrate mucin Stain last only for 2 hours
Fluorescent Acridine Orange Technique (immunofluorescence)
22
Color results for Fluorescent Acridine Orange Technique (immunofluorescence) Acid mucopolysaccharides - Fungi - Background -
Black Greenish red Reddish Orange
23
Stains for Fats and Lipids
Sudan Black Sudan IV (scharlach R) Oil Red O Osmic Acid Stain Nile Blue Sulfate Method Borohydride-Periodic-Schiff Method S2O2 NB
24
Sudan Black Fixative: Section:
Fixative: formaldehyde calcium with post-chroming. Section: cryostat or frozen.
25
Color results for Sudan Black Lipids - Nuclei -
Blue black Red
26
Sudan IV (Scharlach R) Fixative: Section:
Fixative: 10 % formalin Section: frozen
26
Oil Red O Fixative: Section:
Fixative: neutral buffered formalin Section: frozen section
26
Osmic Acid Stain Fixative: Section:
Fixative: 10% formalin Section: cryostat
27
Color results for Oil Red O Fat - Nuclei -
Briilliant Red Blue
27
Color results for Sudan IV (Scharlach R) Lipids (triglycerides) - Nuclei -
Red Blue/Black
28
Used to differentiate type of lipids
Nile Blue Sulfate Method
28
Used as a fixative for EM Not Stain.
Osmic Acid Stain
29
Color results for Osmic Acid Stain Fat - Nuclei -
Black Yellow Orange
30
Nile Blue Sulfate Method Fixative: Section:
10% formalin Frozen
31
Color results for Nile Blue Sulfate Method Neutral Fat - Cholesterin esters and Cholesterin fatty acids - Cerebrosides - Fatty acids and soap -
Pinkish Red Light Red Light Blue Deep blue or violet
32
Stain for gangliosides
Borohydride-Periodic-Schiff Method
33
Borohydride-Periodic-Schiff Method Fixative: Section:
10% formalin Cryostat
34
Color results for Borohydride-Periodic-Schiff Method Gagliosides - Nuclei -
Red Blue
35
Stains for Proteins
Peracetic Acid Alcian Blue Gomori calcium method
36
Stain for Alkaline phosphatase
Gomori calcium method
36
Stain For Cysteine and Cystine.
Peracetic Acid Alcian Blue
37
Gomori calcium method Fixative: Section:
Formol Calcium Cryostat
37
Peracetic Acid Alcian Blue color result
Blue Green
38
Color results for Gomori calcium method ALP activity - Nuclei -
Brownish Black Green
39
Stains for Enzymes
Lead method Calcium-cobalt method a-Napththyl acetate method Indoxyl acetate method Felipe and Lake 1983 Tertazolium method LaC FIT
40
Lead method Fixative: Section:
Formol Calcium Cryostat
41
Stain for 5-nucleotidase
Lead method
42
Stain for esterase.
a-Napththyl Acetate Method and Indoxyle Acetate Method
42
Color result for Lead method
Blackish-brown deposits
43
Metal precipitation. It precipitates phosphatase to demonstrate ATPase.
Calcium-Cobalt Method
44
Color result for Calcium-Cobalt Method
Shades of Brown
45
a-Napththyl Acetate Method Fixative: Section:
Formol Calcium Cryostat
46
Color result for a-Napththyl Acetate Method Esterase - Nuclei -
Reddish Brown Green
47
Stain for Acetylcholinesterase.
Felipe and Lake 1983
48
Color result for Indoxyl Acetate method Ecterase Activity - Nuclei --
Blue Red
49
Color result for Felipe and Lake 1983
Dark brown to Black
49
Nerve cells containing acetylcholinesterase stain
Felipe and Lake 1983
50
For monoamine oxidase Stains bluish black
Tertazolium Method
51
Stains for Nucleic Acids
Feulgen Technique Methyl Green-Pyronin Method Fluorescent staining for DNA and RNA
51
Color result for Tertazolium Method
Bluish Black
52
Stains for Nuclear DNA
Feulgen Technique
53
Color result for Feulgen Technique DNA - Cytoplasm -
Red Purple Green
54
Differential staining for DNA and RNA.
Methyl Green-Pyronin Method
55
Color result for Methyl Green-Pyronin Method DNA - RNA -
Green or Blue Green Rose Red
56
Color result for Flourescent staining for DNA and RNA Rhodamine - Acridine Orange - DNA (___), RNA (____)
Orange red emission Yellow-Green Orange red
57
Connective Tissue Stains For Reticulin
Gomori's Silver Impregnation
57
Color result for Gomori's Silver Impregnation Reticuliin Fibers -
Black
58
Connective Tissue Stains for Collagen
Van Gienson's Stain Masson's Trichrome stain Mallory's Analine Blue Azocarmine Stain
59
Color result for Van Gienson's Stain Collagen -
Pink or deep red
60
Color result for Masson's Trichrome stain Collagen -
Blue
61
CONNECTIVE TISSUE STAIN for elastic fibers
Weigert’s elastic tissue stain- Verhoeff’s elastic method- Orcein (Taenzer-Unna Orcein method)- Krajian’s technique-
62
Color result for Weigert’s elastic tissue stain
dark blue or blue black against dark background.
63
Color result for Verhoeff’s elastic method Elastic Fiber - Collagen Fiber -
Black Red
63
Color result for Orcein (Taenzer-Unna Orcein method)-
Dark brown
64
Color result for Krajian’s technique-
Bright red color
65
Connective tissue stain for Fibrins
MSB Technique PTAH method
66
Connective tissue stains for Amyloid
Gram's Iodine Congo Red Method Metachromatic Staining Induced Fluorescent Staining With Thioflavibne
67
Color result for MSB technique - PTAH Method -
Red Dark Blue
68
Color results for stains for amyloid Gram's Iodine - Congo Red Method - Metachromatic Staining -
Purple or Blue Pink or Red Pinkish Red or Purplish Red
69
Stains for Muscle
Modified Gomori’s Trichrome Stain- Mallory’s PTAH- Heidenhain’s Iron Hematoxylin- Lissamine Fast Red-
70
Color Result for stains for muscle Modified Gomori’s Trichrome Stain- Mallory’s PTAH- Heidenhain’s Iron Hematoxylin- Lissamine Fast Red-
Red Blue Grey-Black Red
71
BONE MARROW AND BLOOD STAINS
Wright stain Giemsa Stain Wright-Giemsa or Jenner Stain May-Grunwald Stain Peroxidase Reaction
72
Color results for Schmorl’s Picro-thionin Method Lacunae and canaliculi - Bone Matrix - Cells -
Dark brown - Black Yellow-brownish yellow Red
73
Stain for for neurons, Axons, Neurofibrils (black against gray background.
Bielschowsky's Technique
74
CENTRAL NERVOUS TISSUE STAIN
Bielschowsky's Technique Bodian's Stain Sevier Munger Technique Cresyl Fast Violet Weigert Pal Technique Luxol Fast Blue
74
for myeloid cells (green or dark blue granules in cytoplasm
Peroxidase Reaction
75
Stain for neural tissues.
Sevier Munger Technique
76
Stain for nerve fibers and nerve endings.
Bodian's Stain
77
stain for Nissl bodies.
Cresyl Fast Violet
78
Stain for Myelin Sheaths.
Weigert Pal Technique
79
stain for H and E stain for Myelin.
Luxol Fast Blue
80
for myelin sheath (black)
Weil’s Method-
81
for astrocytes (black against brownish background).
Cajal’s Gold Sublimate-
82
PIGMENTS AND DEPOSITS stains
Perl's Prussian Blue Turnbull's Blue Reaction Benzidine Nitroprusside Stain Modified Fouchet's Technique Gmelin
83
Stain for for hemosiderin (ferric iron) deep blue color.
Perl’s Prussian Blue
84
Stain for for hemosiderin
Turnbull's Blue Reaction
85
Stain for hemoglobin and oxidase granules
Benzidine Nitroprusside Stain
86
Stain for for bile pigments (emerald- blue green).
Modified Fouchet's Technique
87
Stain for for bile and hematoidi-
Gmelin

Histopath Midterms (4 decks)

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