Special microscopy Flashcards
What is darkfield microscopy?
Darkfield microscopy is used to visualize unstained or transparent specimens.
With this technique, the condenser does not allow light to pass directly through the specimen, but rather the light is directed and an oblique angle. Only light that hits objects in the specimen will be deflected upwards into the objective lens.
What are the advantages of darkfield mycroscopy?
It does not require staining of the specimen. can be used on live specimens and allows visualization of very thin organisms.
What type of microscopy?
No direct rays of light enter the objective lens.
Darkfield
What is a disadvantage of darkfield microscopy?
only provides an image of specimen surfaces that refract the light.
What is fluorescence microscopy?
Uses special filters in a unique method of illumination to produce fluorescent light.
Involves two wave lengths of light.
What is Immunofluorescence?
Fluorochrom - labeled antibodies can be used to label certain parts of a cell.
What is fluorochrome?
Florescent dye.
What is polarizing microscopy used for?
It is used to examine and identify biological structures, including crystals that have unique optical properties. Birefringence.
Polarized light is a contrast enhancing technique allowing better image quality with birefringent materials. Primarily crystals and specimens.
Polarizing prisms or filters are used to allow light to be transmitted in a single plane.
What is a polarizing microscope?
Is a compound light microscope fitted with a polarizer and an analyzer. The polarizer is placed between the light source and the specimen. The analyzer is placed between the specimen and the eye.
What is a polarizing microscope used to examine?
Body fluids for the presence of crystals, especially urine and synovial fluids. Also, it is useful for demonstrating the presence of fat, especially cholesterol in fluids.
What is inverted microscopy?
The term inverted microscopy stems from the reverse positioning of the optics.
The light source and condenser are located above the spessman on the stage, while the oculars and objective lenses are located below the stage. This allows for greater working distance.
What is electron microscopy used for?
Is used to obtain high-resolution images of samples. magnifications of up to 10,000,000x that of a compound microscope.
What are the two types of electron microscopes?
Transmission electron microscope (TEM)
Scanning electron microscope (SEM)
What is digital microscopy?
A digital imaging system consists of a microscope, digital camera computer, and imaging software that has become the mainstay of the hematology and urinalysis laboratories.
