Sources Of Error Flashcards

1
Q

Alcian Blue 2.5

A

Too much AB staining will cause nuclear staining

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2
Q

Colloidal iron

A

The pH of colloidal iron must be below 2.0 otherwise background staining

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3
Q

Congo Red

A

Cut too thin: faint red
Cut too thick: yellow

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4
Q

Masson Trichrome

A

Decreased red stain - solution aged
Decreased blue stain - overdifferentiated in acetic acid

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