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CLINICAL CHEMISTRY LABORATORY > SODIUM > Flashcards

SODIUM Flashcards

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1
Q

What is the major cation of extracellular fluid?

A

Sodium

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2
Q

What are the functions of Sodium in the body?

A

Maintenance of the normal distribution of water
Osmotic pressure in the various fluid compartments

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3
Q

What is the main source of body sodium?

A

Sodium chloride ingested in foods

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4
Q

About how much total body sodium is contained in the skeleton?

A

1/3

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5
Q

Hyponatremia is associated with what conditions?

A

Severe polyuria
Metabolic acidosis
Addison’s disease
Diarrhea
Renal Tubular disease

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6
Q

Hyponatremia is associated with what conditions?

A

Hyperadrenalism
Severe dehydration
Diabetic coma after therapy with insulin
Excess treatment with sodium salts

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7
Q

The present method of sodium colorimetric method is based on modifications of those first described by?

A

Maruna and Trinder

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8
Q

In the first modification of Maruna and Trinder, sodium is precipitated as?

A

Triple salt

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9
Q

Describe the reaction to produce chromophore

A

Sodium magnesium uranyl acetate with the excess Uranium is reacted with ferrocyanide.

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10
Q

What is the filtrate reagent of the sodium colorimetric method?

A

Uranyl Acetate 2.1mM
Magnesium acetate 2.0mM in ethyl alcohol

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11
Q

What is the acid reagent under SCM?

A

A diluted acetic acid

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12
Q

What are the sodium colorimetric method?

A

Potassium ferrocyanide
Non-reactive stabilizers
Filters

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13
Q

What is the sodium standard of the sodium chloride solution?

A

150mEq/L

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14
Q

What is the storage of sodium reagent?

A

15-30C (Room temperature)

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15
Q

The specimen that will be used should be?

A

Freshly drawn serum

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16
Q

What is the stability of sodium?

A

24 hours
2 weeks if refrigerated

17
Q

What are the materials required but not provided?

A

Spectrophotometer
Centrifuge
Test tubes/rack

18
Q

In the laboratory activity, what is the volume of the filtrate reagent?

A

1000uL

19
Q

What is the volume of blk, std and sample added?

A

50 uL

20
Q

After the addition of the filtrate reagent, std, blk, and sample. What should be next?

A

Shake all the tubes vigorously for 3 minutes.

21
Q

What is the RPM of the centrifuge?

A

1500 for 10 minutes

22
Q

What is the volume of the acid reagent added?

A

1000uL

23
Q

What is the volume added of std,blk and sample supernatant?

A

50 uL

24
Q

What is the volume of the color reagent added?

A

50 uL

25
Q

At what absorbance are the tubes read?

A

550nm

26
Q

These elements have levels up to 3X normal and have no adverse influence on the procedure

A

Calcium, Chloride, Potassium

27
Q

This element have level up to 5X normal and have no adverse influence on the procedure

A

Phosphorus

28
Q

Failure to obtain the proper range of values in the assay of control may?

A

Indicate reagent deterioration
Instrument malfunction
Procedural errors

29
Q

Where is the sodium colorimetric compared to?

A

Flame photometric analysis

CLINICAL CHEMISTRY LABORATORY (2 decks)

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