SODIUM Flashcards
What is the major cation of extracellular fluid?
Sodium
What are the functions of Sodium in the body?
Maintenance of the normal distribution of water
Osmotic pressure in the various fluid compartments
What is the main source of body sodium?
Sodium chloride ingested in foods
About how much total body sodium is contained in the skeleton?
1/3
Hyponatremia is associated with what conditions?
Severe polyuria
Metabolic acidosis
Addison’s disease
Diarrhea
Renal Tubular disease
Hyponatremia is associated with what conditions?
Hyperadrenalism
Severe dehydration
Diabetic coma after therapy with insulin
Excess treatment with sodium salts
The present method of sodium colorimetric method is based on modifications of those first described by?
Maruna and Trinder
In the first modification of Maruna and Trinder, sodium is precipitated as?
Triple salt
Describe the reaction to produce chromophore
Sodium magnesium uranyl acetate with the excess Uranium is reacted with ferrocyanide.
What is the filtrate reagent of the sodium colorimetric method?
Uranyl Acetate 2.1mM
Magnesium acetate 2.0mM in ethyl alcohol
What is the acid reagent under SCM?
A diluted acetic acid
What are the sodium colorimetric method?
Potassium ferrocyanide
Non-reactive stabilizers
Filters
What is the sodium standard of the sodium chloride solution?
150mEq/L
What is the storage of sodium reagent?
15-30C (Room temperature)
The specimen that will be used should be?
Freshly drawn serum
What is the stability of sodium?
24 hours
2 weeks if refrigerated
What are the materials required but not provided?
Spectrophotometer
Centrifuge
Test tubes/rack
In the laboratory activity, what is the volume of the filtrate reagent?
1000uL
What is the volume of blk, std and sample added?
50 uL
After the addition of the filtrate reagent, std, blk, and sample. What should be next?
Shake all the tubes vigorously for 3 minutes.
What is the RPM of the centrifuge?
1500 for 10 minutes
What is the volume of the acid reagent added?
1000uL
What is the volume added of std,blk and sample supernatant?
50 uL
What is the volume of the color reagent added?
50 uL
At what absorbance are the tubes read?
550nm
These elements have levels up to 3X normal and have no adverse influence on the procedure
Calcium, Chloride, Potassium
This element have level up to 5X normal and have no adverse influence on the procedure
Phosphorus
Failure to obtain the proper range of values in the assay of control may?
Indicate reagent deterioration
Instrument malfunction
Procedural errors
Where is the sodium colorimetric compared to?
Flame photometric analysis
