POTASSIUM

Question 1

Assay for potassium is intended for?

Answer 1

Colorimetric method determination of POTASSIUM in human serum and plasma

Question 2

What cation is the principle cation of the ICF?

Answer 2

Potassium

Question 3

This is an important constituent of the extracellular fluid due to its influence on muscle activity.

Answer 3

Potassium

Question 4

What are the intracellular functions of Potassium?

Answer 4

Influencing acid-base balance
Osmotic pressure
Water retention

Question 5

Hyperkalemia is often associated with what disease?

Answer 5

Renal failure
Dehydration shock or Adrenal insufficiency

Question 6

Hypokalemia are associated with what diseases?

Answer 6

Malnutrition
Negative nitrogen balance
GIT fluid losses
Hyperactivity of the adrenal cortex

Question 7

The amount of potassium is determined by using?

Answer 7

Sodium tetraphenylboron

Question 8

The amount of potassium is determined by using sodium tetraphenylboron to produce a _________________?

Answer 8

Colloidal suspension

Question 9

What is the relationship of turbidity and potassium concentration in the range of 2-7mEq/L?

Answer 9

Proportional

Question 10

What is the principle of the test utilizing potassium determination?

Answer 10

Turbidimetry

Question 11

What are the potassium regents?

Answer 11

Sodium tetraphenylboron 2.1mM
Preservatives and thickening agents

Question 12

What is the concentration of potassium standard?

Answer 12

Equivalent to 4 mEq/L

Question 13

Potassium reagent is used for this diagnostic only

Answer 13

In vitro

Question 14

What is the storage of potassium reagent and standard?

Answer 14

Potassium reagent: 15 - 30C (room temp)
Potassium standard: 2-8C (ref temp)

Question 15

What is the stability of potassium reagent and standard?

Answer 15

Until expiration date indicated on the package level

Question 16

Potassium reagent should not be used if?

Answer 16

The reagent is very cloudy
The reagent fails to achieve assigned value on fresh control serum.

Question 17

Potassium in serum is stable for at least __________ at ______.

Answer 17

2 weeks at 2-8C

Question 18

TRUE OR FALSE:
Plasma from anticoagulants not containing potassium is not suitable.

Answer 18

False: SUITABLE

Question 19

What are the interferences of the Potassium test?

Answer 19

Turbid or icteric samples
Bilirubin above 40mg/dL
Nitrogen above 80mg/dL
Hemolyzed sera

Question 20

What are the materials required but not approved?

Answer 20

Spectrophotometer
Test tubes/rack
Timer

Question 21

In the laboratory, what is the volume of the potassium reagent added?

Answer 21

1000 uL

Question 22

In the laboratory activity, what is the volume of the sample, blk and std added?

Answer 22

10uL

Question 23

How many minutes are the sample, blk and std sit at room temperature?

Answer 23

3 minutes

Question 24

At what absorbance is the potassium test read?

Answer 24

500nm

Question 25

The usage of this may not produce accurate results.

Answer 25

Potassium calibrator

Question 26

What are the other products that may interfere with the procedure and tend to falsely elevate the results?

Answer 26

Preservatives

Question 27

What should be done if the sample's value is above 7 mEq/L?

Answer 27

It should be diluted with 1:1 with normal saline, re-assayed and multiplied by 2.