POTASSIUM Flashcards

1
Q

Assay for potassium is intended for?

A

Colorimetric method determination of POTASSIUM in human serum and plasma

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2
Q

What cation is the principle cation of the ICF?

A

Potassium

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3
Q

This is an important constituent of the extracellular fluid due to its influence on muscle activity.

A

Potassium

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4
Q

What are the intracellular functions of Potassium?

A

Influencing acid-base balance
Osmotic pressure
Water retention

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5
Q

Hyperkalemia is often associated with what disease?

A

Renal failure
Dehydration shock or Adrenal insufficiency

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6
Q

Hypokalemia are associated with what diseases?

A

Malnutrition
Negative nitrogen balance
GIT fluid losses
Hyperactivity of the adrenal cortex

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7
Q

The amount of potassium is determined by using?

A

Sodium tetraphenylboron

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8
Q

The amount of potassium is determined by using sodium tetraphenylboron to produce a _________________?

A

Colloidal suspension

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9
Q

What is the relationship of turbidity and potassium concentration in the range of 2-7mEq/L?

A

Proportional

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10
Q

What is the principle of the test utilizing potassium determination?

A

Turbidimetry

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11
Q

What are the potassium regents?

A

Sodium tetraphenylboron 2.1mM
Preservatives and thickening agents

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12
Q

What is the concentration of potassium standard?

A

Equivalent to 4 mEq/L

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13
Q

Potassium reagent is used for this diagnostic only

A

In vitro

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14
Q

What is the storage of potassium reagent and standard?

A

Potassium reagent: 15 - 30C (room temp)
Potassium standard: 2-8C (ref temp)

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15
Q

What is the stability of potassium reagent and standard?

A

Until expiration date indicated on the package level

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16
Q

Potassium reagent should not be used if?

A

The reagent is very cloudy
The reagent fails to achieve assigned value on fresh control serum.

17
Q

Potassium in serum is stable for at least __________ at ______.

A

2 weeks at 2-8C

18
Q

TRUE OR FALSE:
Plasma from anticoagulants not containing potassium is not suitable.

A

False: SUITABLE

19
Q

What are the interferences of the Potassium test?

A

Turbid or icteric samples
Bilirubin above 40mg/dL
Nitrogen above 80mg/dL
Hemolyzed sera

20
Q

What are the materials required but not approved?

A

Spectrophotometer
Test tubes/rack
Timer

21
Q

In the laboratory, what is the volume of the potassium reagent added?

A

1000 uL

22
Q

In the laboratory activity, what is the volume of the sample, blk and std added?

A

10uL

23
Q

How many minutes are the sample, blk and std sit at room temperature?

A

3 minutes

24
Q

At what absorbance is the potassium test read?

A

500nm

25
Q

The usage of this may not produce accurate results.

A

Potassium calibrator

26
Q

What are the other products that may interfere with the procedure and tend to falsely elevate the results?

A

Preservatives

27
Q

What should be done if the sample’s value is above 7 mEq/L?

A

It should be diluted with 1:1 with normal saline, re-assayed and multiplied by 2.