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BACTERIOLOGY P2 > Small Pleomorphic Gram Negative Bacilli > Flashcards

Small Pleomorphic Gram Negative Bacilli Flashcards

1
Q

-Cottontail rabbit
– primary reservoir
- both wild and domesticated animals
- birds
- arthropods
- also in water, muds, and animal feces

A

Francisella tularensis

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2
Q

Transmission:
1. Tick bites 3. Ingestion
2. Inhalation 4. Direct

A

Francisella tularensis

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3
Q

Causes Tularemia
Ulceroglandular – at the site of inoculation

A

Francisella tularensis

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4
Q

conjunctivitis, papule of the lower lid, associated lymphadenitis

A

Oculoglandular Francisella tularensis

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5
Q

Typhoidal - Gastrointestinal

Pneumonic Tularemia

A

Francisella tularensis

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6
Q

Typhoidal - Gastrointestinal
Pneumonic Tularemia

A

Francisella tularensis

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7
Q

small pleomorphic gram – negative rods with faint bipolar staining

A

Francisella tularensis

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8
Q

-rarely done
- slow grower (usually within 2 – 4 days) - special growth requirements
i. Glucose – cyteine agar supplemented with 5% defibrinated rabbit blood
ii. Chocolate Agar with IsolVitalX
iii. Buffered charcoal yeast extract agar (BYCE)
iv. Blood agar plate (BAP)

A

Francisella tularensis

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9
Q

Colonies: blue – gray to white round, smooth and slightly mucoid
iv. Blood agar plate (BAP)
- may show small zone of alpha hemolysis

A

Francisella tularensis

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10
Q

Serologic test
– Slide agglutination or Direct fluorescent antibody test (DFAT)
- titer of 1:40 in the absence of previous disease
-Can cross react with Brucella abortus.

A

Francisella tularensis

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11
Q

Not a Facultative Intracellular parasite

A

Pasteurella multocida

Facul-tative anaerobe

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12
Q

Strictly aerobic
Non – motile
Facultative intracellular organisms
Coccoid to pleomorphic rod – shaped

A

Francisella spp.

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13
Q

Pasteurella multocida
Virulence

A

Capsule

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14
Q

Pasteurella multocida
Transmission

A

From dog or cat bites

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15
Q

Local:
wound infections – animal bites or scratch wounds
Respiratory tract infections:
Sinutsitis, peritonsillar abscess, mastoiditis, pulmonary abscess, pneumonia, empyema, bronchitis and
bronchiectasis
- systemic:
septicemia, meningitis,osteomyelitis

A

Pasteurella multocida

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16
Q
  • gram – negative short rod with bipolar staining
    2. Culture:
  • rarely grown on gram
    – negative differential media (EMB or MacConkey)
  • grow on Blood Agar (BAP)may have musty or mousy odor
A

Pasteurella multocida

17
Q

Pasteurella multocida
Biochemical tests:

A

Catalase = +
Indole = +
Oxidase = +
ONPG – Negative Penicillin susceptible

18
Q

most important human pathogen

A

Pasteurella multocida

19
Q

• Facultative anaerobe
• Non – motile
• Range morphologically from coccobacilli to long filamentous rods
• Found as commensals in the upper respiratory tract of fowl and mammals

A

Pasteurella spp.

20
Q

Brucella spp.
Named base on their preferential host

A

B. abortus Cows
B. melitensis Goats
B. suis Pigs
B. canis Dog

21
Q

Obligate aerobe
Facultative intracellular parasite Encapsulated, non – motile Catalase and oxidase positive

A

Brucella spp.

22
Q

Brucella spp.
Virulence Factors

A
  1. Capsule
  2. Tropism to erythritol
23
Q
  1. Direct contact with contaminated livestock or aborted placentas.
  2. Ingestion of infected milk.
  3. Inhalation of organisms.
A

Brucella spp.

24
Q

Bang’s Disease
1. Diurnal fever – “Undulant fever”
2. Non – specific signs and symptoms
- weakness - sweats
- anorexia - malaise
3. Organomegaly – splenomegaly, hepatomegaly and lymphadenopathy
ii. Induces abortion in animals

A

Brucella spp.
Brucellosis/ Bang’s Disease

25
Q

Gram stain
- faintly staining
- Rod to coccoid – shaped, arranged singly or in pairs –“appearance of a sand”

A

Brucella spp.

26
Q

Culture
- slow growing
- growth enhanced by 5 to 10 % CO2
i. Blood Agar Plate (BAP) – incubate for 4- 6weeks
ii. MacConkey Agar
iii. Brucella Agar

A

Brucella spp.

27
Q

all are catalase, oxidase and urease positive

A

Brucella spp.

28
Q

Minimum titer of 1:160 in a standard tube agglutination test

A

Brucella spp.

29
Q

– 4 fold or greater during the the first two months of illness

A

Recent brucellosis

BACTERIOLOGY P2 (3 decks)

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