Slide Preparation

Question 1

Steps in Slide Preparation and Purpose

Answer 1

1. Fixation
   to preserve tissue morphology and architecture, prevent cell digestion
2. Embedding
   obtain rigid consistency for sectioning
3. Staining
   use of dyes to make certain components visible

Question 2

Fixatives used

Answer 2

Formaldehyde

Glutaraldehyde

Question 3

Steps in Embedding (Dehydration)

Answer 3

remove water
increase concentrations of EtOH (40, 60 , 80 , 90%)
water is replaced by alcohol

Question 4

Steps in Embedding (clearing)

Answer 4

bathe lipid solvent (benzene or xylene) remove alcohol
bake in paraffin
sectioned using microtome