siRNA Flashcards
1
Q
What does RNAi stand for?
A
RNA interfernece
2
Q
What does RNAi interfere with?
A
translation
3
Q
What is used to destroy the mRNA before it is translated?
A
small interfering RNA (siRNA)
4
Q
What produces siRNA?
A
an enzyme that cuts large double stranded RNA into smaller sections
5
Q
What does siRNA bind to?
A
an enzyme (Risc)
6
Q
What does the siRNA-enzyme complex do?
A
bind to the mRNA via complementary base pairing
7
Q
How many of the siRNA STRANDS binds to an enzyme?
A
one
8
Q
What happens after siRNA binds to the enzyme?
A
- siRNA molecule guides the enzyme to a mRNA molecule
- due to complementary base pairing
- the enzyme cuts mRNA into smaller sections
9
Q
How does siRNA prevent a gene from being expressed?
A
- one of the siRNA strands binds to enzyme
- guides the enzyme to a specific mRNA molecule, due to complementary base pairing
- the enzyme cuts mRNA into small sections
- preventing translation
