Simulation 4-6 (for Lab Practical One) Flashcards
What technique is used?
Quad streak
The purpose of a streak plate isolation technique is to obtain a pure bacterial culture?
True or false
True
What does CFU stand for?
Colony forming unit
Why do you sterilize the wire loop between streaking each quarter of the plate while streaking for isolation?
A. Reduce the number of bacteria on the wire loop
B. The wire loop needs to be warm to boost growth
C. Prevent an epidemic
D. Avoid contamination
The flame from the Bunsen burner kills all the microbes and sterilizes the wire loop. Why is it important to do before working with a sample?
A. Avoid contamination
B. All the answers are correct
C. Decrease spread of antibiotic resistance
D. Salmonella can be found everywhere
Avoid contamination
In nature, microbes live separately, but are sometimes found in communities of multiple microbial species?
True or false
False
Aseptic technique is essential for successful isolation of microorganisms?
True or false
True
What are the two types of streak plate isolation techniques?
T-Streak and Quad Streak
Bacteria can be quantified, or enumerated using the T-streak method of isolation
True or false
False
Why are bacteria samples best stored in the fridge?
Reduce bacterial growth
Which method can we used to identify if the antibiotic-resistant bacteria in the chicken shed sample are Salmonella?
Grow the bacteria on a salmonella shigella agar plate
Why are the bacteria samples stored in the fridge?
Reduce bacterial growth
The flame kills all microbes and sterilizes the wire loop. Why is this important to do before working with the chicken farm sample?
To avoid contamination
There are black and red antibiotic resistant bacterial species growing on the agar plate. Can you identify these species?
Salmonella and E. Coli
Why is it important for us to isolate a single colony of bacteria from the chicken farm sample?
We require a sample in which all the bacteria cells are genetically identical
What statement best described the purpose of the T-streaking technique
The decrease the concentration of bacterial cells on the agar surface
How can you tell a colony was successfully isolated?
The bacterial colony is deposited few millimeters away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color
Which agar plate resulted from the student not cooling the wire look after sterilization?
Which agar plate resulted from the student not working using aseptic technique?
Which agar plate resulted from the student not sterilizing their wire loop between streaking each sector?
What are the two streak plate methods of isolation
T-Streak Method and Quad- Streak Method
What is the difference between T-Streak method and Quad-streak method?
T-Streak has 3 sections and Quad has 4
What is Spread plate method of isolation?
A method that allows microbiologists to quantify or enumerate the number of viable cells from in the original poly microbial sample
Which setting is best to view the moving bacteria and bacterial shapes?
Dark field mode
Why do bacteria move?
To stay away from harmful environments
To move towards attractive environments
To seek other bacteria to create colonies or biofilms giving additional advantages
What is shape of bacteria
Diplococci
What is bacteria shape?
Spirochete
What is bacteria shape
Vibrio
What is bacteria shape?
Bacillus
What is not eukaryotic or prokaryotic?
Virus
What is the main function of cell wall?
To provide structure and protect the bacteria from osmotic pressure
Both eukaryotic and bacteria contain plasma membranes. Which of the following statements explains the functions of the plasma membrane.
It controls which substances enter and exit the cell
How is the nucleoid involved in helpings the vibrio bacteria survive the harsh environment?
The nucleoid encodes essential genes and for some bacteria these are genes essential for surviving extreme cold
What is the plasmids role in survival in extreme conditions?
They can encode for proteins that provide additional benefits for the bacteria to survive the local environment
How does the ribosome ensure that the vibrio can survive in the extreme environment?
The cell would not exist without as the ribosomes translate the genetic code into protein
How do inclusions bodies assist the bacteria in survival?
They hold nutrients necessary for the bacteria to survive
The cell wall protects the bacteria. How does it protect vibrio in Antarctica?
The withstand the osmotic pressure from the aquatic environment.
