Simulation 4-6 (for Lab Practical One)

Question 1

What technique is used?

Answer 1

Quad streak

Question 2

The purpose of a streak plate isolation technique is to obtain a pure bacterial culture?

True or false

Answer 2

True

Question 3

What does CFU stand for?

Answer 3

Colony forming unit

Question 4

Why do you sterilize the wire loop between streaking each quarter of the plate while streaking for isolation?
A. Reduce the number of bacteria on the wire loop
B. The wire loop needs to be warm to boost growth
C. Prevent an epidemic
D. Avoid contamination

Question 5

The flame from the Bunsen burner kills all the microbes and sterilizes the wire loop. Why is it important to do before working with a sample?

A. Avoid contamination
B. All the answers are correct
C. Decrease spread of antibiotic resistance
D. Salmonella can be found everywhere

Answer 5

Avoid contamination

Question 6

In nature, microbes live separately, but are sometimes found in communities of multiple microbial species?

True or false

Answer 6

False

Question 7

Aseptic technique is essential for successful isolation of microorganisms?

True or false

Answer 7

True

Question 8

What are the two types of streak plate isolation techniques?

Answer 8

T-Streak and Quad Streak

Question 9

Bacteria can be quantified, or enumerated using the T-streak method of isolation

True or false

Answer 9

False

Question 10

Why are bacteria samples best stored in the fridge?

Answer 10

Reduce bacterial growth

Question 11

Which method can we used to identify if the antibiotic-resistant bacteria in the chicken shed sample are Salmonella?

Answer 11

Grow the bacteria on a salmonella shigella agar plate

Question 12

Why are the bacteria samples stored in the fridge?

Answer 12

Reduce bacterial growth

Question 13

The flame kills all microbes and sterilizes the wire loop. Why is this important to do before working with the chicken farm sample?

Answer 13

To avoid contamination

Question 14

There are black and red antibiotic resistant bacterial species growing on the agar plate. Can you identify these species?

Answer 14

Salmonella and E. Coli

Question 15

Why is it important for us to isolate a single colony of bacteria from the chicken farm sample?

Answer 15

We require a sample in which all the bacteria cells are genetically identical

Question 16

What statement best described the purpose of the T-streaking technique

Answer 16

The decrease the concentration of bacterial cells on the agar surface

Question 17

How can you tell a colony was successfully isolated?

Answer 17

The bacterial colony is deposited few millimeters away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color

Question 18

Which agar plate resulted from the student not cooling the wire look after sterilization?

Question 19

Which agar plate resulted from the student not working using aseptic technique?

Question 20

Which agar plate resulted from the student not sterilizing their wire loop between streaking each sector?

Question 21

What are the two streak plate methods of isolation

Answer 21

T-Streak Method and Quad- Streak Method

Question 22

What is the difference between T-Streak method and Quad-streak method?

Answer 22

T-Streak has 3 sections and Quad has 4

Question 23

What is Spread plate method of isolation?

Answer 23

A method that allows microbiologists to quantify or enumerate the number of viable cells from in the original poly microbial sample

Question 24

Which setting is best to view the moving bacteria and bacterial shapes?

Answer 24

Dark field mode

Question 25

Why do bacteria move?

Answer 25

To stay away from harmful environments
To move towards attractive environments
To seek other bacteria to create colonies or biofilms giving additional advantages

Question 26

What is shape of bacteria

Answer 26

Diplococci

Question 27

What is bacteria shape?

Answer 27

Spirochete

Question 28

What is bacteria shape

Answer 28

Vibrio

Question 29

What is bacteria shape?

Answer 29

Bacillus

Question 30

What is not eukaryotic or prokaryotic?

Answer 30

Virus

Question 31

What is the main function of cell wall?

Answer 31

To provide structure and protect the bacteria from osmotic pressure

Question 32

Both eukaryotic and bacteria contain plasma membranes. Which of the following statements explains the functions of the plasma membrane.

Answer 32

It controls which substances enter and exit the cell

Question 33

How is the nucleoid involved in helpings the vibrio bacteria survive the harsh environment?

Answer 33

The nucleoid encodes essential genes and for some bacteria these are genes essential for surviving extreme cold

Question 34

What is the plasmids role in survival in extreme conditions?

Answer 34

They can encode for proteins that provide additional benefits for the bacteria to survive the local environment

Question 35

How does the ribosome ensure that the vibrio can survive in the extreme environment?

Answer 35

The cell would not exist without as the ribosomes translate the genetic code into protein

Question 36

How do inclusions bodies assist the bacteria in survival?

Answer 36

They hold nutrients necessary for the bacteria to survive

Question 37

The cell wall protects the bacteria. How does it protect vibrio in Antarctica?

Answer 37

The withstand the osmotic pressure from the aquatic environment.