Simple Staining and universal aspects of staining

Question 1

What is a universal step of staining?

Answer 1

• heat fixation: various methods used depending on stain procedure; kill organism and fixes it on slide

Question 2

List

Simple Stains

3

Answer 2

1. methylene blue
2. crystal violet
3. safranin

Question 3

Steps of Simple Stain

Answer 3

• heat fix organism
• cover slide with crystal violet for 1 minute
• rinse slide with distilled water
• observe under 100x objective lens

Question 4

Important note with ANY stained slide.

Answer 4

remember what side of the slide has the bacterial stain, this side should remain UP to be viewed directly by the objective lens