Acid-Fast Stain

Question 1

Step 1 of Acid-Fast stain

Answer 1

heat fix organism; place organism on slide prior to water d/t the waxy coating of these bacteria

Question 2

Step 2 of Acid-Fast stain

Answer 2

flood slide w/ carbolfuschin for 2 minutes; rinse w/ distilled water

Question 3

Step 3 of Acid-Fast stain

Answer 3

hold slide at angle and rinse w/ acid alchol until color runs off; rinse w/ distilled water

Question 4

Step 4 of Acid-Fast stain

Answer 4

flood slide w/ methylene blue for 30 seconds; rinse w/ distilled water

Question 5

Acid-Fast stain - important notes for final step

Answer 5

allow to air dry; DO NOT blot w/ paper towel

Question 6

What does the acid-fast stain stain for?

Answer 6

the mycolic acid present in the cell wall of Mycobacterium genus bacteria

Question 7

What color are acid-fast cells?

Answer 7

fuchia/pinkish; the lighter colored cells

Question 8

What color are non-acid-fast cells?

Answer 8

darker purple/blue; the darker colored cells