SHAFTERED INTO THE ER

Question 1

What’s needed for translocation of proteins into ER lumen

Answer 1

SEC61

Question 2

Difference in roles of cis and trans cisternae

Answer 2

Cis: Receiving station for ER-derived vesicle and escaped ER proteins
Trans: Packaging into vesicles at TGN and uses coat protein.

Question 3

Required ER quality control system, 3 needed complexes

Answer 3

Step-wise removal of sugar residues as system to monitor protein folding status 
Complexes:
1) SDP-BIP-ERDj3A complex
2) CNX/CRT cycle
3) PDI system

Question 4

How is IRE1 used in ER stress signalling

Answer 4

IRE1 alternatively splices mRNA of specific bZIP TFs

-When stress is detected, the receptor dimerised and phos

Question 5

How is bZIP28 used in ER stress signalling

Answer 5

ER stress induces bZIP28 (S1P + S2P in golgi) relocation to nucleus to regulate UPR genes

Question 6

3 things needed for plant immunity

Answer 6

Salicylic acid: activator of plant immunity
NPR1: SA receptor
Psm ES4326: Bacterial pathogen of plants

Question 7

Whats in the unfolded protein response (UPR) (mild ER streess)

Answer 7

1) Induction of chaperones
2) Degradation of misfolded proteins
3) Attenuated translation

Question 8

Severe/constant ER stress response

Answer 8

1) ER swelling

2) Caspase-mediated apoptosis