Set 2 - Recent Flashcards
This gives the surface of gram-negative bacteria a negative charge.
A.Mycolic acid
B.Outer membrane
C.Periplasm
D.Lipopolysaccharide
Lipopolysaccharide
The following statement are true except:
A.Gram + bacteria ….peptidoglycan layer.
B.The periplasmic space is found in gram – and gram + bacteria.
C.The capsule of gram + bacteria is immediate to … murein layer.
D.The sex pilus is a conduit for passage of DNA
The periplasmic space is found in gram – and gram + bacteria.
This method of sterilization is applied to substances that are heat-sensitive
A.Filtration
B.Radiation
C.Cold sterilization
D.Oxidation
Filtration
It is used as prophylaxis in newborns to prevent ocular n. gonorrhea infection
A.Erythromycin
B.Silver nitrate
C.Povidone Iodine
D.Vancomycin
A.Erythromycin
Ratio of sodium hypochlorite recommended by CDC for cleaning tabletops after blood spills:
A. 1:5
B. 1:10
C. 1:20
D. NOTA
The following statement are true regarding specimen collection except:
A.Specimen should be collected during acute phase of an illness
B.Serum for serologic studies can be frozen at –70C for up to 1 week
C.N. meningitidis is sensitive to temp changes
D. Processing should be done upon receipt at the lab
B.Serum for serologic studies can be frozen at –70C for up to 1 week
This anticoagulant is commonly used for viral cultures
A.EDTA
B.Citrate
C.SPS
D.Heparin
D.Heparin
This is added to transport media to absorb fatty acids
A.Buffer
B.Antibiotic
C.Charcoal
D.Indicator
C.Charcoal
The following statement are true except
A. Direct microscopy examination is done to all specimens
B. All processing should begin with a gross examination of the specimen
C. Sterile fluid should be given priority for processing
D. Mislabeled specimens should be rejected
A. Direct microscopy examination is done to all specimens
Inhibits the growth of gram + organism in your MacConkey
A.Phenol Red
B.Crystal violet
C.Ferric Ammonium Citrate
D.Lactose
B.Crystal violet
The presence of this enzyme allows the release of water and oxygen from hydrogen peroxide. What process is being described?
A. Oxidase
B. Catalase
C. Oxidation reaction
D. Coagulase
B. Catalase
What is the positive result for Catalase?
A.Bubble formation
B.Purple color
C.Clot formation
D.Reddening
A.Bubble formation
Case JJ 5 yo male polio vax seen due to appearance of bright red rash on his cheeks associated with
low grade fever and nasal discharge What is the causative agent?
A. Enveloped virus with icosahedral nucleocapsid and …. double stranded DNA
B. Naked nucleocapsid with single-stranded positive polarity RNA
C. Enveloped virus with icosahedral nucleocapsid and one piece of single stranded positive polarity RNA
D. Nonenveloped icosahedral single stranded DNA Virus
D. Nonenveloped icosahedral single stranded DNA Virus
Major site of viral replication
A.Capillaries of the skin
B.Bone marrow
C.Placenta
D.Neurons
B.Bone marrow
Major virulence factor for S. aureus
A.Capsule
B.Protein
C.Coagulase
D.Lime layer
C.Coagulase
This test will differentiate your proteus mirabilis from proteus vulgaris
Indole
What is the principle of the INDOLE test?
A.Detects the end products of glucose formation of by your peptidoglycan pathway
B.Detects end products of glucose from the mixed acid pathway
C.Determines the organism has the ability to reduce nitrate to nitrite
D.Test the ability of the organism to produce the enzyme tryptophanase
B.Detects end products of glucose from the mixed acid pathway
TRUE OR FALSE?
Case KK 38 yo male single sexually active came in for consult due to maculopapular
rash on his arms and soles associated with progressive weight loss on and off undocumented fever and occasional malaise. Susceptibility testing can be performed for the causative agent
FALSE(causative agent is syphilis)
When you start treatment what test can you request to follow the patient’s response to therapy
A.RPR
B.FTA-ABS
C.EIA
D.Microscopy
A.RPR
What is the specimen of choice if you are suspecting congenital transmission of the causative
agent?
A.Plasma
B.Serum
C.Placenta
D.Umbilical cord
D.Umbilical cord
The following statement are true except:
A.Chlamydia contains lipopolysaccharide in their cell wall
B.Endocervix the preferred site to collect screening specimens in female patient with chlamydia
C.One of the differences seen in chlamydia is the shape of the reticulate body
D.Humans are the only host of chlamydia pneumoniae
C.One of the differences seen in chlamydia is the shape of the reticulate body
A 35 year old female complains of nonhealing ulcer in her left index finger that now progress to a postular lesion. WHat is the mycelial formation of the fungus?
A. Daisy-head or floweret microconidia
B. Thick-walled yeasts with multiple buds resembling a mariner’s wheel
A. Daisy-head or floweret microconidia
The following statement are true except:
A. If infection with Malassezia furfur is suspected, Olive oil should be added to the medium
B. If processing is delayed dermatologic specimen should be refrigerated
C. Respiratory tract secretions are the most common specimen collected for fungal culture
D. CSF specimens should be processed immediately
B. If processing is delayed dermatologic specimen should be refrigerated
The macroconidia of this organism are rarely formed vary in ssize and shaped and referred to a
string-bean appearance
A.Trichophyton robrum
B.Tonsurans
C.Mentagrophytes
D.Verrucosum
D.Verrucosum
Most sensitive test to diagnose streptococcosis
A.India ink
B.Culture
C.Gram stain
D.Latex agglutination
D.Latex agglutination
This test differentiate Listeria monocytogenes from other listeria species
A. CAMP Test
B. Wet mount
C. Carbohydrate fermentation
A. CAMP Test
What test is the most helpful in the definitive diagnosis of the causative agent
A. Culture
B. Gram stain
C. Demonstration of toxins
D. Demonstration of metachromatic granules
C. Demonstration of toxins
What is being detected by string test for vibrio
A. Capsule
B. Flagella
C. DNA
D. Villi
C. DNA
Which of hte following vibrios are responsible for pandemic?
A.Vibrio cholerae
B.Vulnificus
C.Parahaemolyticus
D.Cholerae O1 biotype El Tor
D.Cholerae O1 biotype El Tor
Presumptive test of the identification of your group a beta hemolytic streptococcus?
A. Hemolysis on blood agar
B. Bacitracin test
C. Novobiocin test
D. Optochin test
B. Bacitracin test
This is known as a microscopic precipitin test in which your cap surrounding the organism will swell?
A.India ink
B.Quellung Test
C.Crystal violet
D.String test
B.Quellung Test
The folloiwng statement are true except
A. MacConkey Agar is a nutritive and differential media
B. Blood agar is a nutritive and differential media
C. TCBS agar is a selective and differential media
D. Modified Thayer martin is an enrichment and selective media
A. MacConkey Agar is a nutritive and differential media
The following statement are true about gram staining except:
A.It is considered a differential stain
B.All bacteria can be detected using this staining method
C. It gives clue about the quality of the specimen
B.All bacteria can be detected using this staining method
The following statement are true except
A.Gram + positive bacteria with have a thick peptidoglycan layer
B.Gram – bacteria have an outer lipid bilayer
C.Gram + bacteria will have an outer lipid layer
D.Gram – bacteria will have a thin peptidoglycan layer
C.Gram + bacteria will have an outer lipid layer
This bacteria structure is most often target of antibiotics:
A. Outer membrane
B. Cell wall
C. Capsule
D. Lipopolysaccharide
B. Cell wall
This test will differentiate streptococcus pneumoniae from alpha hemolytic streptococci
A.PYR test
B.LAP test
C.Bile solubility
D.Bacitracin
C.Bile solubility
What population is at risk for necrotizing enteritis?
A.Newborn
B.Toddlers
C.Unvaccinated children
D.Preterm infants
D.Preterm infants
Specimen of choice for pediatric patient suspected of tb?
A. Sputum
B.Induced sputum
C.Bronchial alveolar lavage
D.Gastric lavage
D.Gastric lavage
The following statement are true regarding chlamydia except:
A.The elementary body is metabolically inert
B.The reticulate body is the infective form
C.The elementary is the extracellular form
B.The reticulate body is the infective form
Which Sero variant of chlamydia can cause ocular trachoma?
A. Ba
B. L1
C. L1A
D. K
A. Ba
Which of the following is transmitted by Chiggers?
A. Rickettsiae
B. Orientia tsutsumagushi
B. Orientia tsutsumagushi
What attenuated strain of mycobacteria is present in the BCG vaccine?
A. M bovis
B. M africanum
C. M leprae
A. M bovis
What type of motility is seen on dark field microscopy of vibrios?
A. Tumbling
B. Corkscrew
C. Darting
D. Shooting star
D. Shooting star
This is added to the medium Modified Thayer Martin agar to inhibit the growth of proteus ..
Trimethoprim
Selective media for isolation of your s. aureus
A. Sheep blood agar
B. Mannitol salt agar
C. Chocolate agar
D. Nutrient broth
B. Mannitol salt agar
Causative agent of your cat’s scratch disease
Bartonella
What is used to seen gastric biopsy for detection of h. pylori?
A. Wright Giemsa
B. Warthin-starry
C. Gram stain
D. H&E
B. Warthin-starry
Cultivation of Ricketssia:
A. Footpads of mice
B. Footpads of armadillos
C. Embryonated egg
C. Embryonated egg
This associated with dental or surgical procedure often leading to tooth abscess
Viridans
Biosafety cabinet offers the most protection
Class III
Mycobacterium tuberculosis is considered what type of biosafety level?
Level III
Preservative used for stool specimen
Polyvinyl alcohol
Exhibits satellite phenomenon
Haemophilus
Double zone of beta-hemolysis
C. perfringes
Causative agent of Yaws:
A. T. pallidum subspecies pallidum
B. T. pallidum subspecies pertenue
B. T. pallidum subspecies pertenue
T or F?
Direct microscopy examination is performed on all specimens
False
T or F?
Malachite green used for acid fast staining
False
T or F?
Slide coagulase test detects clumping factor
True
T or F?
A positive Hippurate hydrolysis test will show yellow color.
False
T or F?
Enterococci is resistant to high salt concentration.
True
T or F?
Cutaneous diphtheriae is caused by non-toxigenic strains only.
False
T or F?
Blood Culture for typhoid fever is positive during hte 1st week of illness.
True
T or F?
Haemophilus is very sensitive to drying and extreme temperatures.
True
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Complications of mumps.
1.Parotitis
2.Meningitis
3.Swelling
4.Orchitis
B (2&4)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Toxin-mediated diseases of s.areus.
1.Food poisoning
2.Ritter syndrome
3Toxic shock syndrome
4.Folliculitis
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Specimen suitable for anaerobic culture.
1.Pleural fluid
2.Blood
3.CSF
4.Expectorated sputum
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
A positive gram stain with a negative culture may indicate.
1.Poor transport methods
2.Old culture media
3.Excess exposure to air during processing
4.Poor medtech skills
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Specimens used for the diagnosis of mucormycosis.
1.Tissue
2.Sputum
3.Nasal discharge
4.Blood
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Causes bloody diarrhea.
1.S. aureus
2.Enteroinvasise E.coli
3….
4Shigella
B (2&4)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Produces odor in culture.
1.Proteus
2.Eikenella
3.Pseudomonas
4.C. difficile
E (AOTA)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Normal microbiota in humans.
1.S. aureus
2.H. influenzae
3.S. agalactiae
4.C.diptheria
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Types of Fire Extinguisher.
1.A
2.B
3.C
4.D
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Anticoagulant used in the microbio lab.
1.SPS
2.EDTA
3.Heparin
4.Citrate
A (1&3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Rejection of unacceptable specimens.
1.Vaginal swab for anaerobic culture
2.Foley-catheter tips for culture
3.Nasopharyngeal for anaerobic culture
4.Specimen was received in fixative
E (AOTA)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Viruses with cytoplasmic inclusion bodies.
1.Rabies
2.CMV
3.Poxvirus
4.Herpes simplex virus
A (1&3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
This can cause subacute endocarditis.
1.S. aureus
2.Eikenella
3.Kingella
4.S. epidermidis
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Characteristics of nocardia.
1.Gram +
2.Saprophyte
3.Aerobic
4.Partially acid fast
E (AOTA)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Can cause UTI.
1.P. aeruginosa
2.E. coli
3.P. mirabilis
4.S. saprophyticus
E (AOTA)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Vertical Transmission.
1.Syphilis
2.Hep b
3.L. monocytogenes
4.S. aureus
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Colors of the fire diamond.
1.Red
2.Yellow
3.Blue
4.Green
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Specimens that do not require decontamination for the recovery of mycobateria.
1.Sputum
2.Joint fluid
3.Urine
4.CSF
B (2&4)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Symptoms of rickettsia.
1.Fever
2.Lympadenopathy
3.Rash
4.Headache
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Sexually transmitted.
1.N. gonorrhaea
2.K. granumolatis
3.C. trachomatis
4.H. ducreyi
E (AOTA)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Mycobacteria that occur in humans that is capable of causing tuberculosis.
1.M. bovis
2.M. tuberculosis
3.M. africanum
4.M. kansasi
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Symptoms of congenital syphilis.
1.Saber shin
2.Deafness
3….Maxilla
4.Blindness
B (2&4)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Swarming colonies.
1.P. mirabilis
2.C. tetani
3.P. vulgari
4.S. pneumoniae
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Necessary for the growth of heamophilus.
1.NAD
2.V Factor
3.Hemin
4.X Factor
E (AOTA)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Causes of bacterial meningitis.
1.N. meningitidis
2.S. pneumoniae
3.H. influenzae
4.K. pneumoniae
C (1,2,3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Symptoms of scarlet fever.
1.Rash
2.Lymphadenopathy
3.Strawberry tongue
4.Honey-crust lesion
A (1&3)
A. 1&3; B. 2&4; C. 1,2,3; D. 4 only; E. AOTA
Naturally inhabitant of the human vagina.
1.Candida
2.G. vaginalis
3.Neisseria
4.Lactobacillus
B (2&4)
What is the positive reaction of Indole Test?
A.Red color
B.Growth in the medium with color change
C.Turbidity
D.Bright pink color
A.Red color
The following statement are true regarding viruses except:
A.All DNA viruses are icosahedral except poxvirus
B.All RNA viruses reticate(?) in the cytoplasm
C.Herpesviridae has the ability to establish latency
D.Poxvirus is the largest and the most complex of all viruses
B.All RNA viruses reticate(?) in the cytoplasm