Serological Testing Flashcards
What is a non treponemal test for serologic detection of the antibody Reagin?
Rapid Plasma Reagin
Patients infected with Treponema pallidum produce this phospholipid:
Reagin
Syphilis infected patients will have this phospholipid in their serum/plasma:
Reagin
what is in the antigen in the RPR test?
Carbon Charcoal particle
Is RPR screening or confirmatory?
screening
if flocculation is present on the RPR, this is indicative of a positive or a negative test?
reactive, positive
What is the confirmatory test for syphilis?
Fluorescent treponemal antibody absorbed test (FTA-ABS)
how will you know if your FTS-ABS is positive?
fluoresce if positive
two types of tubes for RPR test?
Red or Purple
how do you know if the serum is acceptable for testing with RPR?
clear and non hemolyzed
steps prior to testing RPR:
centrifuge the speciment
mix suspension, shaking for 10-15 seconds
attach needle to dispensing bottle
draw all the antigen into dispensing bottle
how many drops of specimen do you apply to the RPR card?
1 drop
how long should the RPR test be on the rotator and and how many RPM’s?
8 minutes, @ 100 RPM
what is the method for differentiating non reactive from weakly reactive results?
3 or 4 to- and fro- motion
how are results recorded on the RPR test?
either reactive or non reactive
flocculation ranges on a reactive RPR test:
slight but definite
minimum to moderate
marked and intense
What is the next step if you have a reactive RPR test?
confirmed with FTA-ABS
What are some essentials to start with for proper Quality Control of an RPR?
proper collection and handling of specimen, assaying control reagents, and checking instrument performance.
what are the two sections of Quality Control?
Equipment QC
Reagent QC
What is the mechanical rotator calibrated to?
100 rpm
what is the antigen needle calibrated to?
60 drops per 1 mL
what will you run every time an unknown sample is tested?
control reagents (reactive, weak reactive, and non reactive)
Which virus causes infectious mononucleosis?
Epstein Barr Virus
What test is used for testing for mono?
monospot
what is population that is more prone to mono?
young adults and children less than 5 years old.
SX of mono:
fever, malaise, lethargy, sore throat with exudates, lymphadenopathy, mild hepatitis, splenagomalia, skin rash
what are the names of the abnormal lymphocytes and monocytes in the lymph nodes that are structurally similar to the antibody that is specifically produces as a response to antigen stimulation?
heterophile
you may test the specimen for Monospot test within 24 hrs in which condition?
is stored between 2-8 degrees Celsius
Latex reagent and controls should be at what temperature prior to testing Monospot?
room temp. 20-30 degrees C
How should the Latex reagent for Monospot be shaken prior to testing?
gently. 10-15 seconds
how long should both single drops of both sample and reagent be rotated?
3 minutes manually or on a rotator shaker set at 60-100 rpm
How are results reported for Monospot?
either positive or negative
How does a negative result appear on a Monospot?
smooth, homogenous solution with no agglutination.
