Serological Testing

Question 1

What is a non treponemal test for serologic detection of the antibody Reagin?

Answer 1

Rapid Plasma Reagin

Question 2

Patients infected with Treponema pallidum produce this phospholipid:

Answer 2

Reagin

Question 3

Syphilis infected patients will have this phospholipid in their serum/plasma:

Answer 3

Reagin

Question 4

what is in the antigen in the RPR test?

Answer 4

Carbon Charcoal particle

Question 5

Is RPR screening or confirmatory?

Answer 5

screening

Question 6

if flocculation is present on the RPR, this is indicative of a positive or a negative test?

Answer 6

reactive, positive

Question 7

What is the confirmatory test for syphilis?

Answer 7

Fluorescent treponemal antibody absorbed test (FTA-ABS)

Question 8

how will you know if your FTS-ABS is positive?

Answer 8

fluoresce if positive

Question 9

two types of tubes for RPR test?

Answer 9

Red or Purple

Question 10

how do you know if the serum is acceptable for testing with RPR?

Answer 10

clear and non hemolyzed

Question 11

steps prior to testing RPR:

Answer 11

centrifuge the speciment
mix suspension, shaking for 10-15 seconds
attach needle to dispensing bottle
draw all the antigen into dispensing bottle

Question 12

how many drops of specimen do you apply to the RPR card?

Answer 12

1 drop

Question 13

how long should the RPR test be on the rotator and and how many RPM’s?

Answer 13

8 minutes, @ 100 RPM

Question 14

what is the method for differentiating non reactive from weakly reactive results?

Answer 14

3 or 4 to- and fro- motion

Question 15

how are results recorded on the RPR test?

Answer 15

either reactive or non reactive

Question 16

flocculation ranges on a reactive RPR test:

Answer 16

slight but definite
minimum to moderate
marked and intense

Question 17

What is the next step if you have a reactive RPR test?

Answer 17

confirmed with FTA-ABS

Question 18

What are some essentials to start with for proper Quality Control of an RPR?

Answer 18

proper collection and handling of specimen, assaying control reagents, and checking instrument performance.

Question 19

what are the two sections of Quality Control?

Answer 19

Equipment QC

Reagent QC

Question 20

What is the mechanical rotator calibrated to?

Answer 20

100 rpm

Question 21

what is the antigen needle calibrated to?

Answer 21

60 drops per 1 mL

Question 22

what will you run every time an unknown sample is tested?

Answer 22

control reagents (reactive, weak reactive, and non reactive)

Question 23

Which virus causes infectious mononucleosis?

Answer 23

Epstein Barr Virus

Question 24

What test is used for testing for mono?

Answer 24

monospot

Question 25

what is population that is more prone to mono?

Answer 25

young adults and children less than 5 years old.

Question 26

SX of mono:

Answer 26

fever, malaise, lethargy, sore throat with exudates, lymphadenopathy, mild hepatitis, splenagomalia, skin rash

Question 27

what are the names of the abnormal lymphocytes and monocytes in the lymph nodes that are structurally similar to the antibody that is specifically produces as a response to antigen stimulation?

Answer 27

heterophile

Question 28

you may test the specimen for Monospot test within 24 hrs in which condition?

Answer 28

is stored between 2-8 degrees Celsius

Question 29

Latex reagent and controls should be at what temperature prior to testing Monospot?

Answer 29

room temp. 20-30 degrees C

Question 30

How should the Latex reagent for Monospot be shaken prior to testing?

Answer 30

gently. 10-15 seconds

Question 31

how long should both single drops of both sample and reagent be rotated?

Answer 31

3 minutes manually or on a rotator shaker set at 60-100 rpm

Question 32

How are results reported for Monospot?

Answer 32

either positive or negative

Question 33

How does a negative result appear on a Monospot?

Answer 33

smooth, homogenous solution with no agglutination.