Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

IMSE > Serological Diagnosis of infectious diseases > Flashcards

Serological Diagnosis of infectious diseases Flashcards

1
Q

immunity is [against/pro] to M protein

A

Against

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

a M protein infectious diease that is immune-serotype specific.

A

Steptococcus Pyogenes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Pathological consequences in Streoptococcus Pyogenees

A

Skin
Upper respiratory infection
Rheumatic fever
Post-streptococcal Glomerulonephritis**

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Antibodies against strep such as m protein, cross-react w/ myocardial tissue leading to this pathological consequence

A

rheumatic fever

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Most diagnostically important antibodies

A

Anti-streptolysin O
AntiDNase B
Anti-NADase
Anti- Hyaluronidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Assays used for the detection of STREPTOCOCCAL ANTIGENS

A

[CELMS]
Cultivation
Enzyme Immunoassay
Latex Agglutination
Molecular Diagnostics
SEROTYPING

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

It is an assay where there is m proteins precipitation by _________ antisera.

PROS:
CONS:

A

Serotyping
Type-specific

fast turnaround time
new m type proteins not recognized =difficulty in results interpretation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Assays used for the detection of STREPTOCOCCAL ANTIBODIES [explain]
– recommendation: at least ____per pax

A

2

Anti-Streptolysin O testing [ASO, Nephelometry]
1.ASO Test
Diluted px’s serum + Streptolysin O + RBC indicator

Results: titer in todd units [reciprocal of highest dilution demonstrating no hemolysis]

2.Nephelometry

Anti-DNAse B testing
1.Neutralization Methodology [classic]
Diluted px’s serum+ DNAse B+ Methyl green conjugate

Results: 0-4+ [highest dilution reciprocal] [2 up w/ color]

2.Nephelometry

  1. Streptozyme testing
    Px’s serum + Sheep RBC coated w/ strepto-lysin/kinase, DNAse, NADase, Hyaluronidase

Dilution 1:100

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

assay used for the detection of streptococcal antibodies that is clinically useful for suspected glomerulonephritis by streptococcal infections

A

Anti-DNAse B testing [Neutralization, Nephelometry]

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

gram negative microaerophilic spiral bacterium

A

Helicobacter Pylori

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

major cause of gastric and duodenal ulcers

A

Helicobacter Pylori

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Assays used for the detection of HELICOBACTER PYLORI ANTIGENS

A

[endoscopy, -EMA]
Bacteria Cultivation
Gastric Biopsy tissue
Ureasse Biopsy tissue
Urea Breath testing

Enzyme Immunoassay [bacterial ag in feces]
Molecular tests [H. pylori DNA]
AB tests

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

which of the assays used for the detection of h. pylori antigens require the use of endoscopy?

A

Bacteria cultivation
Gastric Biopsy
Ureasse Biopsy tissue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

which of the assays used for the detection of h. pylori antigens do not require the use of endoscopy?

A

Urea Breath testing
EIA [bacterial ag in feces]
Molecular test [h/pylori DNA]
Ab tests

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Assays used for the detection of HELICOBACTER PYLORI ANTIBODIES

A

Ab Classes [IgG, IgA, IgM] – most used to least
ELISA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

an infectious disease that lacks cell wall

A

Mycobacterium pneumoniae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Assays used for the detection of MYCOBACTERIUM PNEUMONIAE ANTIBODIES

A

[PIC-EM]
Particle agglutination
Indirect immunofluorescence
COMPLEMENT fixation

EIA
Molecular technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

most widely used method of antibodies detection in m. pneumoniae

A

EIA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

used assay before but not anymore now

A

cold agglutinins [ab]

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Pathological consequence of m.pneumoniae

A

Upper respiratory infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

2 distinct groups of Rickettsia [elaborate]

A
  1. Spotted fever group
    rickettsii: Rocky mountain spotted fever, tick bite
    japonica: Japanese spotted fever, tick bite
    felis: Flea-borne spotted fever, unknown
    akari: Rickettsialpox, mite bite
  2. Typus Group
    typhi: Endemic typhus, flea feces
    prowazekii: Epidemic typhus, louse feces
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

rickettsia are transmitted via? [+examples]

humans are accidental hosts for rickettsiae EXCEPT ____ [why?]

A

arthropods [ticks, mite, flea, lice]

prowazekii, epidemic typhus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Assays used for the detection of RICKETTSIAL ANTIBODIES

A

[well-felix, III, -EM]
Well- Felix test

Indirect fluorescent assay [IFA]
Immunoblot assays [IBA]
Immunoperoxidase assays [IPA]
Microimmunofluorescent assays [Micro-IF]

ELISA
Molecular techniques

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Well-felix test as an assay used for the detection of rickettsial [Ab/Ag] is based on the cross-[reactivity/linking] of antibodies of rickettsial species AGAINST what? [3]

A

Ab

reactivity

Polysaccharide Antigens of OX-19
Proteus vulgaris of OX-2
Proteus mirabilis of OX-K

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
From the weil-felx reaction test, which of the rickettsial species are positive for? [elaborate]
1+ OX-2: rickettsia, prowazekii, typhi 4+ OX-19: rickettsia, prowazekii, typhi 4+ OX-K: tsutsugamushi
26
Syphilis is also called as
Great Pox Evil Pox French disease Italian disease Spanish disease
27
Syphilis is caused by?
Treponema Pallidum
28
________: It is rapidly destroyed by heat, cold, and drying out-- so they are always spread via ______.
Syphilis direct contact
29
Syphilis has a [few/many] proteins on cell surface that's why it is [easy/hard] to kill by the immune system.
few proteins hard
30
Lesions of : 1.Primary 2.Secondary 3.Latent 4.Tertiary
1. Hard chance 2. Condylomata lata 3. none 4. Gumma, neurosyphilis, CVD
31
which of the types of syphilis are positive in dark field microscopy utilization?
Primary syphilis Secondary syphilis
32
which of the types of syphilis are positive in serological test utilization?
Secondary syphilis Latent Syphilis Tertiatry Syphilis
33
primary body defense against treponemal invasion
intact skin mucous membranes
34
Assays used for the detection of SPIROCHETES [syphilis] w/ the use of what sample?
serous fluid from lesion 1. Non-Treponemal Test [VDRL,RPR, FALSE-POSITIVE] 2. Treponemal Test [FTA-ABS, TPI, TPHA, MHA-TP, Hemeagglutination Treponemal Test for Syphilis, Molecular Diagnostics]
35
____: type of direct detection of spirochetes where reagin , an antibody to ____, is used for detection
Non-treponemal test cardiolipin
36
NON-TREPONEMAL VDRL - - - Read [micro/macro]scopically IF CSF, we use ______. Result: Non reactive-Weakly Reactive-Reactive = [elaborate]
Glass slide Hamilton Syringe Microscopically Boemer agglutination slides Non-reactive: no clumps, slight roughness Weakly reactive: small clumps Reactive: medium-large clumps
37
Sample with weakly reactive-reactive results in VDRL Non-Treponemal test, must be tested using ___________. In which, initially, there are 2-fold dilutions of serum: _______ to _______. Sera yielding positive results for ______ dilution are titered further.
Quantitative Slide Test 1:2-1:32 1:32
38
a modification of VDRL Non-Treponemal Test that is [less/more] sensitive
Rapid Plasma Reagin [RPR] More
39
RPR - - -Read [micro/macro]scopically
Plastic-coated disposable card Capillary tube or Dispestir device Macroscopically
40
False-Positive Non-Treponemal Test's other diseases [+sub]
IM LE Malaria Leprosy T. pallidum sub---- pertenue: aws endemicum: bejel caratenum: pinta
41
used as confirmatory test for direct detection of spirochetes since it is harder to perfom
Treponemal Test [FTA-ABS, TPI, TPHA, MHA-TP, Hemeagglutination Treponemal Test for Syphilis, Molecular diagnostics]
42
Results in: 1.FTA-ABS test 2.Treponema Pallidum Immobilization test
1. FTA-ABS 0+ - 4+ [1+ minimally reactive, 2+ reactive --up] 2. TPI Doubtful: 20%-50% organism immobilized Negative: <20% Positive: >50%
43
1+ minimally reactive result in TPI should be REPEATED after _____ [days/weeks]
1-2 WEEKS
44
Treatment for Syphilis
Penicilin Crystalline/Procaine penicilin [for neurosyphilis] Doxocycline
45
Lyme disease Caused by: Transmission: Clinical hallmark of early rash infection: Brain involvement:
Borrelia Burgdorferi Vector borne [tick] Erythema migrans [EM] Neuroborreliosis
46
Assays used for the detection of LYME DISEASE CDC recommends [#] tiered approach
1. IFA or EIA 2. Western Blot -- confirmation
47
Salmonella Caused by: Transmission
Salmonelle enterica sub typhi Contaminated food ingestion
48
Assays used for the detection of SALMONELLA
1. Culture Method 2. Widal Test
49
Widal test for Salmonella detection -- detects antibodies on px' serum against ________, _______, _______. Reagent: for typhoid fever: _______ for tularemia & brucellosis: ______ Results: 0+ - 4+ [elaborate]
typhoid fever, tularemia, brucellosis O and H antigens Brucella abotus 0+- no agglutination 1+- 25% 2+- 50% 3+- 75% 4+- 100% agglutination
50
Also called as: 1. Infectious Hepatitis 2. Post-transfusion Hepatitis 3. Waterborne Hepatitis
Hepatitis A Hepatitis C Hepatitis E
51
INC. during HBV acute infection INC. during HBV chronic infection
HbS AG HbE AG
52
INDICATOR OF 1. active infection 2. virus' active REPLICATION + high infectivity degree 3. CURRENT ACUTE infection 4. RECOVERING from HBV infection
1. HbS AG 2. HbE AG 3. HbC AB 4. HbE AB
53
First HBV marker to appear
HbS AG
54
HBV marker that is not detectable in serum because the _____ _____ masks it. Detectable ONLY through ____
HbC AG viral envelope biopsy
55
Antibody persists for years + provide proactive immunity
HbS AB
56
Hepatitis A: Family: [DNA/RNA] _____ Transmission: Progression to chronic: Complications:
RNA Picor--navridiae Fecal, oral NO [LOW RISK]Fulminant liver disease
57
HBV Lab diagnosis
EIA [Enzyme Immunoassay] CIA [Chemiluminiscent immunoassay]
58
Hepatitis B: Family: [DNA/RNA] _____ Transmission: Progression to chronic: Complications:
DNA Hepad--naviridiae Parenteral, sexual, perinatal YES CHRONIC HEPATITIS, liver cirrhosis Hepatocellular carcinoma
59
Hepatitis C: Family: [DNA/RNA] _____ Transmission: Progression to chronic: Complications:
RNA Flavivi-ridae Parenteral, sexual, perinatal YES Cirrhosis Hepatocellular carcinoma AUTOIMMUNE manifestations
60
HCV Lab diagnosis
[SCREENING] HCV IgG Antibody detection 1. 3rd Generation EIA 2. CIA [CONFIRMATORY] 1. Recombinant Immunoblot Assay [RIBA]
61
Hepatitis D: --can only occur in the presence of hepatitits __ either as _______ or ________. Family: [DNA/RNA] _____ Transmission: Progression to chronic: Complications:
B Coinfection, Superinfection RNA DELTAVIRUS Parenteral #, Perinatal, HBV infection required YES FULMINANT hepatitis, Cirrhosis Hepatocellular carcinoma
62
Confirmatory test for HCV where it detects antibodies to different hcv antigens that have been immobilized into a _________.
Recombinant immunoblot Assay [RIBA] nitrocellulose strip
63
HDV Lab diagnosis
ELISA PCR
64
HDV lab diagnosis [what assay] 1.Anti-HDV Detection 2.RNA detection
1. ELISA 2. PCR
65
Hepatitis E: Family: [DNA/RNA] _____ Transmission: Progression to chronic: Complications:
RNA Hepe-viridae Fecal, oral NO Fulminant liver failure in PREGNANT WOMEN
66
HEV Lab Diagnosis
ELISA/Western Blot/Fluorescent Assay PCR

IMSE (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions