Serological Diagnosis of infectious diseases Flashcards
immunity is [against/pro] to M protein
Against
a M protein infectious diease that is immune-serotype specific.
Steptococcus Pyogenes
Pathological consequences in Streoptococcus Pyogenees
Skin
Upper respiratory infection
Rheumatic fever
Post-streptococcal Glomerulonephritis**
Antibodies against strep such as m protein, cross-react w/ myocardial tissue leading to this pathological consequence
rheumatic fever
Most diagnostically important antibodies
Anti-streptolysin O
AntiDNase B
Anti-NADase
Anti- Hyaluronidase
Assays used for the detection of STREPTOCOCCAL ANTIGENS
[CELMS]
Cultivation
Enzyme Immunoassay
Latex Agglutination
Molecular Diagnostics
SEROTYPING
It is an assay where there is m proteins precipitation by _________ antisera.
PROS:
CONS:
Serotyping
Type-specific
fast turnaround time
new m type proteins not recognized =difficulty in results interpretation
Assays used for the detection of STREPTOCOCCAL ANTIBODIES [explain]
– recommendation: at least ____per pax
2
Anti-Streptolysin O testing [ASO, Nephelometry]
1.ASO Test
Diluted px’s serum + Streptolysin O + RBC indicator
Results: titer in todd units [reciprocal of highest dilution demonstrating no hemolysis]
2.Nephelometry
Anti-DNAse B testing
1.Neutralization Methodology [classic]
Diluted px’s serum+ DNAse B+ Methyl green conjugate
Results: 0-4+ [highest dilution reciprocal] [2 up w/ color]
2.Nephelometry
- Streptozyme testing
Px’s serum + Sheep RBC coated w/ strepto-lysin/kinase, DNAse, NADase, Hyaluronidase
Dilution 1:100
assay used for the detection of streptococcal antibodies that is clinically useful for suspected glomerulonephritis by streptococcal infections
Anti-DNAse B testing [Neutralization, Nephelometry]
gram negative microaerophilic spiral bacterium
Helicobacter Pylori
major cause of gastric and duodenal ulcers
Helicobacter Pylori
Assays used for the detection of HELICOBACTER PYLORI ANTIGENS
[endoscopy, -EMA]
Bacteria Cultivation
Gastric Biopsy tissue
Ureasse Biopsy tissue
Urea Breath testing
Enzyme Immunoassay [bacterial ag in feces]
Molecular tests [H. pylori DNA]
AB tests
which of the assays used for the detection of h. pylori antigens require the use of endoscopy?
Bacteria cultivation
Gastric Biopsy
Ureasse Biopsy tissue
which of the assays used for the detection of h. pylori antigens do not require the use of endoscopy?
Urea Breath testing
EIA [bacterial ag in feces]
Molecular test [h/pylori DNA]
Ab tests
Assays used for the detection of HELICOBACTER PYLORI ANTIBODIES
Ab Classes [IgG, IgA, IgM] – most used to least
ELISA
an infectious disease that lacks cell wall
Mycobacterium pneumoniae
Assays used for the detection of MYCOBACTERIUM PNEUMONIAE ANTIBODIES
[PIC-EM]
Particle agglutination
Indirect immunofluorescence
COMPLEMENT fixation
EIA
Molecular technique
most widely used method of antibodies detection in m. pneumoniae
EIA
used assay before but not anymore now
cold agglutinins [ab]
Pathological consequence of m.pneumoniae
Upper respiratory infection
2 distinct groups of Rickettsia [elaborate]
- Spotted fever group
rickettsii: Rocky mountain spotted fever, tick bite
japonica: Japanese spotted fever, tick bite
felis: Flea-borne spotted fever, unknown
akari: Rickettsialpox, mite bite - Typus Group
typhi: Endemic typhus, flea feces
prowazekii: Epidemic typhus, louse feces
rickettsia are transmitted via? [+examples]
humans are accidental hosts for rickettsiae EXCEPT ____ [why?]
arthropods [ticks, mite, flea, lice]
prowazekii, epidemic typhus
Assays used for the detection of RICKETTSIAL ANTIBODIES
[well-felix, III, -EM]
Well- Felix test
Indirect fluorescent assay [IFA]
Immunoblot assays [IBA]
Immunoperoxidase assays [IPA]
Microimmunofluorescent assays [Micro-IF]
ELISA
Molecular techniques
Well-felix test as an assay used for the detection of rickettsial [Ab/Ag] is based on the cross-[reactivity/linking] of antibodies of rickettsial species AGAINST what? [3]
Ab
reactivity
Polysaccharide Antigens of OX-19
Proteus vulgaris of OX-2
Proteus mirabilis of OX-K
From the weil-felx reaction test, which of the rickettsial species are positive for? [elaborate]
1+ OX-2: rickettsia, prowazekii, typhi
4+ OX-19: rickettsia, prowazekii, typhi
4+ OX-K: tsutsugamushi
Syphilis is also called as
Great Pox
Evil Pox
French disease
Italian disease
Spanish disease