Serological Assays

Question 1

Serum

Answer 1

Liquid portion of the blood remaining after clotting factors are removed

Question 2

Plasma

Answer 2

Liquid portion of blood that still contains clotting factors (factors have been inactivated)

Question 3

Epitope

Answer 3

Antigenic determinant. Specific site on antigen where antibody/TCR binds

Question 4

Paratope

Answer 4

Site on antibody/TCR that binds to the epitope

Question 5

Kinds of Serological Assays

Answer 5

Agglutination, precipitation, solid phase, immunofluorescence

Question 6

Agglutination reactions

Answer 6

Must have at least 2 binding sites, antigen is an insoluble particle. Positive has granulations. Used for blood types

Question 7

Heterophile Antibody Monospot Test

Answer 7

Looking for different loving antibodies. Are not specific for the disease. Used for testing mono.

Question 8

How are blood types named?

Answer 8

Named for the presence of the A and B carbohydrates on the cells. Presence or absence of Rh(D) antigen on RBC is + or -

Question 9

Zeta potential

Answer 9

Repulsive forces between negatively charged RBCs prevent agglutination by IgG antibodies

Question 10

Coombs Test

Answer 10

Adding coombs antibody allows zeta potential to bee overcome, and cells agglutinate.

Question 11

Erythroblastosis fetalis

Answer 11

Mom is Rh -, baby is +. Once 1st baby is born, mom’s body produces antibodies to Rh+, so second child will be attacked by mom’s antibodies.